A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep.
A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep.
复制标题
用于牛和羊的肌内脂肪百分比的基因表达估计器。
DOI：
10.1186/2049-1891-5-35
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发表时间：
2014
影响因子：
7
通讯作者：
Dalrymple BP
中科院分区：
文献类型：
--
作者：
Guo B;Kongsuwan K;Greenwood PL;Zhou G;Zhang W;Dalrymple BP
牛肌肉中甘油三酯和脂肪酸合成与储存相关蛋白质的基因表达与肌内脂肪（IMF）%相关。相同的基因也与绵羊肌肉中的IMF%相关吗？相同的基因组可以用来估计两个物种的IMF%吗？计算20只绵羊背最长肌（LM）基因表达（微阵列）与IMF%之间的相关性。对该数据集与一个等效牛相关数据集和一个牛差异表达数据集进行了综合分析。在牛和羊中，总共鉴定出30个基因与IMF%强相关。基因的重叠是高度显著的，TAG基因集中的13个基因中的8个和FA基因集中的13个基因中的8个分别在与绵羊IMF%最相关的前100个和前500个基因中，P值= 0。在这30个基因中，CIDEA、THRSP、ACSM 1、DGAT 2和FABP 4在两个物种中的平均排序最高。使用来自两个小组的婆罗门牛（对照和激素生长促进剂处理[已知降低肌肉中的IMF%]）和总共22头动物的数据，检查了IMF%的直接测量和不同估计值（超声和基因表达）区分两组的效用。直接测量的IMF%和超声扫描估计的IMF%不能区分两组。然而，使用基因表达估计IMF%区分两组。将用于估计IMF%的基因数量从1增加到5显著增加了辨别力;但将基因数量增加到15导致几乎没有进一步的改善。我们已经证明了一种比较方法的实用性，以确定稳健的估计IMF%的LM在牛和羊。我们还证明了许多方法（可能适用于比传统方法小得多的动物组），使用基因表达对单个农场/处理中的动物IMF%进行排名，或估计两个农场/处理之间IMF%的差异。
The expression of genes encoding proteins involved in triacyglyceride and fatty acid synthesis and storage in cattle muscle are correlated with intramuscular fat (IMF)%. Are the same genes also correlated with IMF% in sheep muscle, and can the same set of genes be used to estimate IMF% in both species? The correlation between gene expression (microarray) and IMF% in the longissimus muscle (LM) of twenty sheep was calculated. An integrated analysis of this dataset with an equivalent cattle correlation dataset and a cattle differential expression dataset was undertaken. A total of 30 genes were identified to be strongly correlated with IMF% in both cattle and sheep. The overlap of genes was highly significant, 8 of the 13 genes in the TAG gene set and 8 of the 13 genes in the FA gene set were in the top 100 and 500 genes respectively most correlated with IMF% in sheep, P-value = 0. Of the 30 genes, CIDEA, THRSP, ACSM1, DGAT2 and FABP4 had the highest average rank in both species. Using the data from two small groups of Brahman cattle (control and Hormone growth promotant-treated [known to decrease IMF% in muscle]) and 22 animals in total, the utility of a direct measure and different estimators of IMF% (ultrasound and gene expression) to differentiate between the two groups were examined. Directly measured IMF% and IMF% estimated from ultrasound scanning could not discriminate between the two groups. However, using gene expression to estimate IMF% discriminated between the two groups. Increasing the number of genes used to estimate IMF% from one to five significantly increased the discrimination power; but increasing the number of genes to 15 resulted in little further improvement. We have demonstrated the utility of a comparative approach to identify robust estimators of IMF% in the LM in cattle and sheep. We have also demonstrated a number of approaches (potentially applicable to much smaller groups of animals than conventional methods) to using gene expression to rank animals for IMF% within a single farm/treatment, or to estimate differences in IMF% between two farms/treatments.
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影响因子：
3.3
作者：
Jeong, J.;Kwon, E. G.;Baik, M.
通讯作者：
Baik, M.
影响因子：
7.1
作者：
Dervishi, E.;Serrano, C.;Calvo, J. H.
通讯作者：
Calvo, J. H.
影响因子：
3.3
作者：
De Jager, N.;Hudson, N. J.;Dalrymple, B. P.
通讯作者：
Dalrymple, B. P.
影响因子：
2.1
作者：
Kongsuwan, K.;Knox, M. R.;Dalrymple, B. P.
通讯作者：
Dalrymple, B. P.
影响因子：
1.4
作者：
Warner, R. D.;Jacob, R. H.;Pethick, D. W.
通讯作者：
Pethick, D. W.