Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress.
Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress.
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南方水稻黑条矮缩病毒感染和温度胁迫下白背飞虱的代谢组变化
DOI：
10.3390/v10070344
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发表时间：
2018-06-26
期刊：
影响因子：
--
通讯作者：
Zhou G
中科院分区：
文献类型：
--
作者：
Zhang T;Feng W;Ye J;Li Z;Zhou G
南方水稻黑条矮缩病毒（SRBSDV）是一种在东亚和东南亚新出现的毁灭性水稻呼肠孤病毒，由长途迁徙害虫白背飞虱（WBPH）传播。我们之前表明，SRBSDV 感染降低了其载体 WBPH 的耐寒性，但提高了耐热性。使用比较代谢组学分析来探索温度应激反应这些变化背后的潜在机制。用 SRBSDV 和/或极端温度应激处理第四代 WBPH 若虫，并使用气相色谱-飞行时间-质谱法进行分析。在病毒感染、冷或热应激后，WBPH 中总共鉴定出 605 个可区分的峰，并分别鉴定出 165、207 和 202 个差异积累的代谢物。核酸和脂肪酸是SRBSDV感染调节的主要代谢物类别，而温度胁迫则调节三羧酸循环化合物、糖和多元醇。对于感染 SRBSDV 并受到温度胁迫的 WBPH 样品，氨基酸、糖和多元醇是最重要的调节代谢物。代谢组学研究表明，SRBSDV可能通过调节昆虫体内氨基酸、糖和多元醇的积累来影响WBPH的极端温度耐受性。
Southern rice black-streaked dwarf virus (SRBSDV) is a devastating newly emerged rice reovirus in Eastern and Southeastern Asia transmitted by a long-distance migratory pest, the white-backed planthopper (WBPH). We previously showed that SRBSDV infection decreased the cold tolerance but improved the heat tolerance of its vector, WBPH. Comparative metabolomic analysis was used to explore the potential mechanisms underlying these changes in temperature stress response. Fourth-generation WBPH nymphs were treated with SRBSDV and/or extreme temperature stress and were analyzed using gas chromatography-time of flight-mass spectrometry. A total of 605 distinguishable peaks were identified and 165, 207, and 202 differentially accumulated metabolites were identified in WBPH after virus infection, cold, or heat stress, respectively. The nucleic acids and fatty acids were the major categories of metabolites regulated by SRBSDV infection, whereas temperature stress regulated tricarboxylic acid cycle compounds, sugars, and polyols. For the WBPH samples infected with SRBSDV and subjected to temperature stress, amino acids, sugars, and polyols were the most significant regulated metabolites. The metabolomics study suggests that SRBSDV may influence the extreme temperature tolerance of WBPH by regulating the accumulation of amino acids, sugars, and polyols in the insect body.
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影响因子：
3.7
作者：
Bahar MH;Hegedus D;Soroka J;Coutu C;Bekkaoui D;Dosdall L
通讯作者：
Dosdall L
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影响因子：
4.6
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Tu Z;Ling B;Xu D;Zhang M;Zhou G
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2.7
作者：
Belliure, Belen;Janssen, Arne;Sabelis, Maurice W.
通讯作者：
Sabelis, Maurice W.
影响因子：
5.2
作者：
Coleman-Derr D;Tringe SG
通讯作者：
Tringe SG