Viral Ejection Proteins: Mosaically Conserved, Conformational Gymnasts.
Viral Ejection Proteins: Mosaically Conserved, Conformational Gymnasts.
复制标题
DOI：
10.3390/microorganisms10030504
复制
发表时间：
2022-02-24
期刊：
影响因子：
4.5
通讯作者：
Cingolani G
中科院分区：
文献类型：
--
作者：
Swanson NA;Hou CD;Cingolani G
细菌病毒(或噬菌体)已经开发出强大的方法来在细菌内传递它们的遗传信息，克服了细菌细胞包膜的复杂性。在Podoviridae超家族的短尾噬菌体中，基因组弹射是由一组神秘的内部病毒粒子蛋白介导的，也被称为弹射或引导蛋白，这是传染性所必需的。由于其可塑性结构和瞬时组装，喷射蛋白的研究具有挑战性，而且与噬菌体外壳蛋白或终止酶亚基等经典成分相比，它们的特征仍然较少。然而，最近大量的低温EM结构阐明了这些蛋白质的组装和构象体操的关键特征，这些组装和构象体操伴随着它们通过门户蛋白通道从病毒粒子头部排出进入宿主。在这篇综述中，我们将使用噬菌体T7为中心的方法，批判性地回顾关于喷射蛋白的文献，破译T7喷射蛋白在喷射前和喷射后的构象变化，并预测这些蛋白在其他Podoviridae中的保守性。挑战在于将排出蛋白的结构与基因组排出的机制联系起来，这是极其复杂的，并使用宿主的机制。
Bacterial viruses (or bacteriophages) have developed formidable ways to deliver their genetic information inside bacteria, overcoming the complexity of the bacterial-cell envelope. In short-tailed phages of the Podoviridae superfamily, genome ejection is mediated by a set of mysterious internal virion proteins, also called ejection or pilot proteins, which are required for infectivity. The ejection proteins are challenging to study due to their plastic structures and transient assembly and have remained less characterized than classical components such as the phage coat protein or terminase subunit. However, a spate of recent cryo-EM structures has elucidated key features underscoring these proteins’ assembly and conformational gymnastics that accompany their expulsion from the virion head through the portal protein channel into the host. In this review, we will use a phage-T7-centric approach to critically review the status of the literature on ejection proteins, decipher the conformational changes of T7 ejection proteins in the pre- and post-ejection conformation, and predict the conservation of these proteins in other Podoviridae. The challenge is to relate the structure of the ejection proteins to the mechanisms of genome ejection, which are exceedingly complex and use the host’s machinery.
登录
查看更多内容
影响因子：
3.2
作者：
GARCIA, LR;MOLINEUX, IJ
通讯作者：
MOLINEUX, IJ
DOI：
10.1073/pnas.1215563110
发表时间：
2013-04-23
影响因子：
11.1
作者：
Guo, Fei;Liu, Zheng;Jiang, Wen
通讯作者：
Jiang, Wen
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.1231887
发表时间：
2013-02-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hu B;Margolin W;Molineux IJ;Liu J
通讯作者：
Liu J
影响因子：
3.7
作者：
Chang, Chung-Yu;Kemp, Priscilla;Molineux, Ian J.
通讯作者：
Molineux, Ian J.