Anticancer Activity of Anthopleura anjunae Oligopeptides in Prostate Cancer DU-145 Cells.
Anticancer Activity of Anthopleura anjunae Oligopeptides in Prostate Cancer DU-145 Cells.
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Anthopleura anjunae 寡肽对前列腺癌 DU-145 细胞的抗癌活性
DOI：
10.3390/md16040125
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发表时间：
2018-04-12
期刊：
影响因子：
5.4
通讯作者：
Chen R
中科院分区：
文献类型：
--
作者：
Wu ZZ;Ding GF;Huang FF;Yang ZS;Yu FM;Tang YP;Jia YL;Zheng YY;Chen R
Ananthopleura anjunae抗肿瘤肽（AAP-H）是从海葵Anthopleura anjunae中提取的一种五肽，其氨基酸序列为Tyr-Val-Pro-Gly-Pro。在这项研究中，我们研究了AAP-H对前列腺癌DU-145细胞增殖的抑制作用，使用甲基噻唑基二苯基四唑溴化物测定。通过苏木精-伊红染色、吖啶橙子/溴化乙锭荧光染色、Hoechst 33258荧光染色和扫描电镜分析细胞形态。JC-1染色后用流式细胞仪测定线粒体膜电位。采用Annexin V-异硫氰酸荧光素和碘化丙啶染色法检测细胞凋亡率，流式细胞仪检测细胞凋亡相关蛋白的表达。结果表明，AAP-H以剂量依赖性和时间依赖性方式诱导活细胞数量显著减少并增加细胞死亡，在24 h、48 h和72 h的IC 50分别约为9.605 mM、7.910 mM和2.298 mM。观察AAP-H处理后凋亡细胞的形态学特征。AAP-H处理后，线粒体膜电位明显降低，细胞凋亡增加。促凋亡蛋白如Bax、细胞色素C、caspase-3和caspase-9增加，而Bcl-2减少。提示AAP-H对前列腺癌DU-145细胞有一定的抑制作用，其机制可能与细胞凋亡有关。因此，AAP-H是一种候选的抗前列腺癌药物或保健食品。
Anthopleura anjunae anti-tumor peptide (AAP-H) is a pentapeptide from the sea anemone Anthopleura anjunae with an amino acid sequence of Tyr-Val-Pro-Gly-Pro that is obtained by alkaline protease enzymatic hydrolysis extraction. In this study, we investigated the inhibitory effects of AAP-H on prostate cancer DU-145 cell proliferation using a methylthiazolyldiphenyl-tetrazolium bromide assay. Cell morphology was analyzed by hematoxylin-eosin staining, acridine orange/ethidium bromide fluorescence staining, Hoechst 33258 fluorescence staining, and scanning electron microscopy. The mitochondrial membrane potential was determined by flow cytometry following JC-1 staining. The cell apoptosis rate was measured by Annexin V-fluorescein isothiocyanate and propidium iodide staining followed by flow cytometric analysis, and the expression of apoptosis-associated proteins was assayed by Western blotting. The results demonstrated that AAP-H induced significant reductions in the number of viable cells and increased cell death in both a dose-dependent and time-dependent manner, with an IC50 of approximately 9.605 mM, 7.910 mM, and 2.298 mM at 24 h, 48 h, and 72 h, respectively. The morphologic characteristics of apoptotic cells were observed after treatment with AAP-H. The mitochondrial membrane potential was markedly decreased, and apoptosis increased after AAP-H treatment. Pro-apoptotic proteins, such as Bax, cytochrome-C, caspase-3, and caspase-9 were increased, but Bcl-2 was decreased. These findings suggest that AAP-H has moderate inhibitory effects on prostate cancer DU-145 cells, and the mechanism might involve the mitochondria-mediated apoptotic pathway. Therefore, AAP-H is a candidate anti-prostate cancer drug or health-care food.
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影响因子：
5.4
作者：
Ma J;Huang F;Lin H;Wang X
通讯作者：
Wang X
影响因子：
--
作者：
Brentnall M;Rodriguez-Menocal L;De Guevara RL;Cepero E;Boise LH
通讯作者：
Boise LH
影响因子：
4.1
作者：
ARUOMA, OI;LAUGHTON, MJ;HALLIWELL, B
通讯作者：
HALLIWELL, B
影响因子：
6.1
作者：
Gherghi, IC;Girousi, ST;Tzimou-Tsitouridou, R
通讯作者：
Tzimou-Tsitouridou, R
影响因子：
7.2
作者：
Overbeeke, R;Yildirim, M;Vermes, I
通讯作者：
Vermes, I