Comparative genome analysis of a norovirus GII.4 strain GZ2013-L10 isolated from South China
Comparative genome analysis of a norovirus GII.4 strain GZ2013-L10 isolated from South China
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华南地区诺如病毒GII.4株GZ2013-L10的比较基因组分析
DOI：
10.1007/s11262-015-1283-9
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发表时间：
2016-01
期刊：
影响因子：
1.6
通讯作者：
Guo, Weipeng
中科院分区：
文献类型：
--
作者：
Wu, Qingping;Kou, Xiaoxia;Zhang, Jumei;Guo, Weipeng
本研究对从我国南方分离的一株诺如病毒GII. 4株的基因组序列进行了比较分析。菌株GZ 2013-L10的RNA基因组由7513个核苷酸组成。基于3个ORF的系统发育分析证实其基因型为GII.Pe/GII. 4 -2012。与其他22个相同变异体的基因组相比，在新基因组中发现了9个不同的核苷酸替换，导致3个氨基酸变化。还收集了GII.4-2012变体的所有138个衣壳蛋白VP 1序列，并且多重比对揭示了35个可变密码子。对GII.4-2012变体与先前的大流行GII.4变体进行了进化分析，在表位A和E（E368，T413）上鉴定出2个独特的变化，这导致其溶剂可及表面积的明显变化。因此，广泛表征了GZ 2013-L10的基因组，并且预测病毒表位上新出现的变异有助于NoV在人类中的持久性。
In this study, the genome sequence of a norovirus GII.4 strain isolated from South China was comparatively analyzed. The RNA genome of the strain GZ2013-L10 was composed of 7513 nucleotides. Phylogenetic analyses based on three ORFs confirmed its genotype as GII.Pe/GII.4-2012. Compared with other 22 genomes of the same variant, nine distinct nucleotide substitutions were found in the new genome, which resulted in three amino acid changes. All 138 capsid protein VP1 sequences of GII.4-2012 variants were also collected, and multiple alignments revealed 35 variable codons. Evolutionary analyses of GII.4-2012 variants were performed against previous pandemic GII.4 variants, and 2 distinctive changes were identified on epitopes A and E (E368, T413), which resulted in an obvious variation of their solvent-accessible surface areas. Therefore, the genome of GZ2013-L10 was extensively characterized, and new emerging variations on viral epitopes were predicted to contribute to NoV persistence in humans.
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影响因子：
3.2
作者：
Jia, Li-ping;Qian, Yuan;Dong, Hui-jin
通讯作者：
Dong, Hui-jin
DOI：
10.2807/ese.18.01.20345-en
发表时间：
2013-01
期刊：
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
影响因子：
--
作者：
J. VanBeek;K. Ambert‐Balay;N. Botteldoorn;John-Sebastian Eden;J. Fonager;J. Hewitt;N. Iritani;
通讯作者：
J. VanBeek;K. Ambert‐Balay;N. Botteldoorn;John-Sebastian Eden;J. Fonager;J. Hewitt;N. Iritani;
影响因子：
5.4
作者：
Lindesmith, Lisa C.;Debbink, Kari;Donaldson, Eric F.
通讯作者：
Donaldson, Eric F.
影响因子：
8.8
作者：
Sabria, Aurora;Pinto, Rosa M.;Guix, Susana
通讯作者：
Guix, Susana
影响因子：
56.9
作者：
Prasad, BVV;Hardy, ME;Estes, MK
通讯作者：
Estes, MK