Calcium movement and membrane potential changes in the early phase of neutrophil activation by phorbol myristate acetate: a study with ion-selective electrodes.
Calcium movement and membrane potential changes in the early phase of neutrophil activation by phorbol myristate acetate: a study with ion-selective electrodes.
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DOI：
10.1083/jcb.93.1.129
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发表时间：
1982-04
期刊：
影响因子：
--
通讯作者：
Romeo D
中科院分区：
文献类型：
--
作者：
Mottola C;Romeo D
为了定量刺激中性粒细胞中的钙运动和膜电位变化，我们通过非常灵敏的离子选择性电极系统测量了Ca 2+和亲脂性阳离子四苯基磷的净通量。3 × 10（-8）M佛波醇12-肉豆蔻酸酯，13-乙酸酯激活中性粒细胞，诱导约20%的总细胞钙释放，初始滞后期小于10 s。Ca 2+外流显着减少ATP耗竭的细胞和钙调素抑制剂的存在下，从而表明它发生的ATP驱动的中性粒细胞质膜的Ca 2+泵的激活。中性粒细胞的活化还诱导培养基45 Ca与细胞钙的交换短暂增加，这在细胞暴露于刺激物后几秒钟可测量，并在约40 s达到峰值。在达到四苯基磷的稳态积累（静息电位为-67 mV）后刺激嗜中性粒细胞导致显著的去极化，滞后期约为60 s。去极化的速率和程度分别降低了40%和65%，在低Na+介质中，但不改性的跨膜阴离子交换抑制剂。高K+培养基使中性粒细胞去极化，而不改变其静息氧化代谢或损害佛波酯的刺激性。佛波醇12-肉豆蔻酸酯也对中性粒细胞的氧化代谢没有影响，不会诱导Ca 2+挤出和膜电位变化。Ca 2+动员，膜电位的变化和激活中性粒细胞功能之间的因果关系进行了讨论。
To quantitate calcium movements and membrane potential changes in stimulated neutrophils, we have measured net fluxes of Ca2+ and of the lipophilic cation tetraphenyl phosphonium by a very sensitive ion- selective electrode system. Activation of neutrophils by 3 X 10(-8) M phorbol 12-myristate, 13-acetate induces a release of approximately 20% of total cell calcium, with an initial lag period of less than 10 s. The Ca2+ outflux is markedly reduced in ATP-depleted cells and in the presence of a calmodulin inhibitor, thereby suggesting that it occurs by activation of the ATP-driven Ca2+ pump of the neutrophil plasmalemma. Activation of neutrophils also induces a transiently increased exchange of medium 45Ca with cell calcium, which is measurable a few seconds after cell exposure to the stimulant and peaks at approximately 40 s. Stimulation of neutrophils after attainment of steady-state accumulation of tetraphenyl phosphonium (resting potential of -67 mV) results in a marked depolarization, with a lag period of approximately 60 s. The rate and extent of depolarization are reduced by 40 and 65%, respectively, in a low Na+ medium but are not modified by an inhibitor of anion exchange across membranes. A high-K+ medium depolarizes neutrophils without either modifying their resting oxidative metabolism or impairing stimulability by the phorbol ester. Phorbol 12-myristate, which also exhibits no effect on the oxidative metabolism of neutrophils, does not induce Ca2+ extrusion and membrane potential changes. The causal relationship between Ca2+ mobilization, membrane potential changes and activation of neutrophil functions is discussed.
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DOI：
10.1083/jcb.82.2.369
发表时间：
1979-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cramer EB;Gallin JI
通讯作者：
Gallin JI
影响因子：
56.9
作者：
BOUCEK, MM;SNYDERMAN, R
通讯作者：
SNYDERMAN, R
DOI：
10.1073/pnas.77.5.2721
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KORCHAK, HM;EISENSTAT, BA;WEISSMANN, G
通讯作者：
WEISSMANN, G
影响因子：
64.8
作者：
ESTENSEN, RD;HILL, HR;GOLDBERG, ND
通讯作者：
GOLDBERG, ND
DOI：
10.1016/0005-2736(73)90389-1
发表时间：
1973-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
HORVATH, C;SOVAK, M
通讯作者：
SOVAK, M