Retention of insulin-like growth factor I bioactivity during the fabrication of sintered polymeric scaffolds.
Retention of insulin-like growth factor I bioactivity during the fabrication of sintered polymeric scaffolds.
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DOI：
10.1088/1748-6041/9/2/025015
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Puleo DA
中科院分区：
文献类型：
--
作者：
Clark A;Milbrandt TA;Hilt JZ;Puleo DA
在组织工程中使用生长因子为软骨再生提供了额外的好处。生长因子，例如胰岛素样生长因子 I (IGF-I)，可以增加细胞增殖，因此可以减少软骨组织再生所需的时间。在这项研究中，IGF-I 从聚乳酸-乙醇酸 (PLGA) 支架中释放出来，该支架的设计目的是减少通常与组织工程支架相关的突发释放。该支架由负载 IGF-I 的 PLGA 微球通过双乳液 (W1/O/W2) 技术制成。然后将微球压缩、在 49°C 下烧结并进行盐浸出。在37、43、45、49和60°C热处理后验证了可溶性IGF-I的生物活性。此外，IGF-I 从烧结支架中释放后的生物活性得到了证实。三相释放持续120天，导致在第1-3天、4-58天和59-120天分别释放20%、55%和25%的IGF-I。将骨髓细胞直接接种到负载 IGF-I 的支架上，根据 DNA 含量显示细胞增殖增加，从而导致糖胺聚糖 (GAG) 产量增加。目前的结果表明，IGF-I 在掺入热处理支架后仍保持活性，进一步增强组织再生的可能性。
The use of growth factors in tissue engineering offers an added benefit to cartilage regeneration. Growth factors, such as insulin-like growth factor I (IGF-I), increase cell proliferation and can therefore decrease the time it takes for cartilage tissue to regrow. In this study, IGF-I was released from poly(lactic-co-glycolic) acid (PLGA) scaffolds that were designed to have a decreased burst release often associated with tissue engineering scaffolds. The scaffolds were fabricated from IGF-I-loaded PLGA microspheres by a double emulsion (W1/O/W2) technique. The microspheres were then compressed, sintered at 49°C, and salt leached. The bioactivity of soluble IGF-I was verified after being heat treated at 37, 43, 45, 49, and 60°C. Additionally, the bioactivity of IGF-I was confirmed after being released from the sintered scaffolds. The triphasic release lasted 120 days resulting in 20%, 55% and 25% of the IGF-I being released during days 1-3, 4-58, and 59-120, respectively. Seeding bone marrow cells directly onto the IGF-I loaded scaffolds showed an increase in cell proliferation, based on DNA content, leading to an increased glycosaminoglycan (GAG) production. The present results demonstrated that IGF-I remains active after being incorporated into heat-treated scaffolds, further enhancing tissue regeneration possibilities.
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