Involvement of rho p21 and its inhibitory GDP/GTP exchange protein (rho GDI) in cell motility
Involvement of rho p21 and its inhibitory GDP/GTP exchange protein (rho GDI) in cell motility
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rho p21 及其抑制性 GDP/GTP 交换蛋白 (rho GDI) 参与细胞运动
DOI：
10.1128/mcb.13.1.72-79.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Y. Takai
中科院分区：
文献类型：
--
作者：
K. Takaishi;A. Kikuchi;S. Kuroda;Kei Kotani;T. Sasaki;Y. Takai
越来越多的证据表明，Rho p21，一种与ras p21相关的小GTP结合蛋白(G蛋白)，调节着肌动球蛋白系统。肌动球蛋白系统被认为是细胞运动所必需的。在本研究中，我们研究了Rho p21、其抑制GDP/GTP交换蛋白(命名为Rho GDI)、其刺激性GDP/GTP交换蛋白(命名为SMG GDS)和肉毒梭菌ADP-核糖基转移酶C3在瑞士3T3细胞运动(趋化运动)中的作用。我们通过吞噬运动测量细胞轨迹来量化细胞的运动能力。向瑞士3T3细胞内微量注射结合GTP-γS的活性形式的RhoA p21或SMG GDS不影响细胞的运动，但向细胞内注射Rho GDI则抑制细胞的运动。将Rho GDI与GTP-γS结合形式的RhoA p21共显微注射可阻止Rho GDI的这种作用，但不能与相同形式的RhoA p21共注射，该RhoA p21缺少C端三个氨基酸，未经脂类后修饰。Ki-rasVal-12 p21或任何GTPγ-S结合形式的小GTP结合蛋白，包括Rac1 p21、G25K和SMG p21B，都不能阻止Rho GDI的作用。在这些小G蛋白中，RhoA p21、rac1 p21和G25K是已知的Rho GDI的底物。微量注射Rho GDI和SMG GDS不能阻止Rho GDI的作用。向瑞士3T3细胞微量注射C3也抑制了细胞的运动。这些结果表明，Rho GDI-Rho p21系统可能通过肌动球蛋白系统调节细胞运动。
Evidence is accumulating that rho p21, a ras p21-related small GTP-binding protein (G protein), regulates the actomyosin system. The actomyosin system is known to be essential for cell motility. In the present study, we examined the action of rho p21, its inhibitory GDP/GTP exchange protein (named rho GDI), its stimulatory GDP/GTP exchange protein (named smg GDS), and Clostridium botulinum ADP-ribosyltransferase C3, known to selectively ADP-ribosylate rho p21 and to impair its function, in cell motility (chemokinesis) of Swiss 3T3 cells. We quantitated the capacity of cell motility by measuring cell tracks by phagokinesis. Microinjection of the GTP gamma S-bound active form of rhoA p21 or smg GDS into Swiss 3T3 cells did not affect cell motility, but microinjection of rho GDI into the cells did inhibit cell motility. This rho GDI action was prevented by comicroinjection of rho GDI with the GTP gamma S-bound form of rhoA p21 but not with the same form of rhoA p21 lacking the C-terminal three amino acids which was not posttranslationally modified with lipids. The rho GDI action was not prevented by Ki-rasVal-12 p21 or any of the GTP gamma S-bound form of other small GTP-binding proteins including rac1 p21, G25K, and smg p21B. Among these small G proteins, rhoA p21, rac1 p21, and G25K are known to be substrates for rho GDI. The rho GDI action was not prevented by comicroinjection of rho GDI with smg GDS. Microinjection of C3 into Swiss 3T3 cells also inhibited cell motility. These results indicate that the rho GDI-rho p21 system regulates cell motility, presumably through the actomyosin system.
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影响因子：
16.6
作者：
M. Barbacid
通讯作者：
M. Barbacid
当肉毒杆菌外切酶 C3 ADP 核糖基化时，21 千道尔顿 G 蛋白的功能修饰。
影响因子：
5.3
作者：
Rubin,EJ;Gill,DM;Boquet,P;Popoff,MR
通讯作者：
Popoff,MR
影响因子：
4.1
作者：
Gibson, RM;Wilson-Delfosse, AL
通讯作者：
Wilson-Delfosse, AL
影响因子：
56.9
作者：
BARSAGI, D;FERAMISCO, JR
通讯作者：
FERAMISCO, JR
影响因子：
56.9
作者：
KNAUS, UG;HEYWORTH, PG;BOKOCH, GM
通讯作者：
BOKOCH, GM