Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats.
Cannabinoid receptor 2 is increased in acutely and chronically inflamed bladder of rats.
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DOI：
10.1016/j.neulet.2008.08.076
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发表时间：
2008-11-07
影响因子：
2.5
通讯作者：
Bjorling, Dale E.
中科院分区：
文献类型：
--
作者：
Merriam, Fabiola Voznika;Wang, Zun-yi;Guerios, Simone Domit;Bjorling, Dale E.
大麻素受体 1 和 2（CB1 和 CB2）是 G 蛋白偶联受体，在全身表达。大麻素受体在膀胱中表达，可能影响膀胱功能。这项研究的目的有两个：确认膀胱、L6/S1 脊髓和背根神经节 (DRG) 中大麻素受体的存在，并确定急性和慢性膀胱炎症对大麻素受体表达的影响。通过膀胱内给予丙烯醛诱导大鼠急性或慢性膀胱炎症。分别使用免疫印迹和定量实时 PCR 测定 CB1 和 CB2 蛋白及其各自 mRNA 的丰度。我们证实了膀胱、L6-S 脊髓和 DRG 中存在 CB1 和 CB2 受体蛋白和 mRNA。急性膀胱炎症诱导膀胱逼尿肌中 CB2 蛋白表达增加，但不增加 CB1 蛋白表达。慢性膀胱炎症增加膀胱CB2蛋白和mRNA的表达，但不增加CB1蛋白或mRNA的表达。脊髓或 DRG 中 CB1 或 CB2 的表达不受急性或慢性膀胱炎症的影响。 CB1和CB2受体存在于膀胱及其相关神经支配中，并且急性或慢性炎症后CB2受体在膀胱中上调。 CB2 受体可能是膀胱炎症和相关疼痛药物治疗的可行靶点。
Cannabinoid receptors 1 and 2 (CB1 and CB2) are G-protein coupled receptors that are expressed throughout the body. Cannabinoid receptor are expressed in the urinary bladder and may affect bladder function. The purpose of this study was twofold: to confirm the presence of cannabinoid receptors in the bladder, the L6/ S1 spinal cord, and dorsal root ganglia (DRG), and to determine the effects of acute and chronic bladder inflammation on expression of cannabinoid receptors. Acute or chronic bladder inflammation was induced in rats by intravesical administration of acrolein. Abundance of CB1 and CB2 protein and their respective mRNA was determined using immunoblotting and quantitative real-time PCR, respectively. We confirmed the presence of CB1 and CB2 receptor protein and mRNA in bladder, L6-S spinal cord, and DRG. Acute bladder inflammation induced increased expression of CB2, but not CB1, protein in the bladder detrusor. Chronic bladder inflammation increased expression of bladder CB2 protein and mRNA but not CB1 protein or mRNA. Expression of CB1 or CB2 in spinal cord or DRG was unaffected by acute or chronic bladder inflammation. CB1 and CB2 receptors are present in the bladder and its associated innervation, and CB2 receptors are up-regulated in bladder after acute or chronic inflammation. CB2 receptors may be a viable target for pharmacological treatment of bladder inflammation and associated pain.
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影响因子：
7.4
作者：
Mitrirattanakul, Somsak;Ramakul, Navapoln;Spigelman, Igor
通讯作者：
Spigelman, Igor
影响因子：
1.7
作者：
Oddiah, D;Anand, P;Rattray, M
通讯作者：
Rattray, M
DOI：
10.1073/pnas.92.8.3376
发表时间：
1995-04-11
影响因子：
11.1
作者：
FACCI, L;DALTOSO, R;LEON, A
通讯作者：
LEON, A
影响因子：
8.8
作者：
Farquhar-Smith, WP;Rice, ASC
通讯作者：
Rice, ASC
影响因子：
2.5
作者：
Jaggar, SI;Sellaturay, S;Rice, ASC
通讯作者：
Rice, ASC