A MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis.
A MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis.
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MYB4-MAN3-甘露糖-MNB1信号级联调节拟南芥的镉耐受性
DOI：
10.1371/journal.pgen.1009636
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发表时间：
2021-06
期刊：
影响因子：
4.5
通讯作者：
Cao S
中科院分区：
文献类型：
--
作者：
Yan X;Huang Y;Song H;Chen F;Geng Q;Hu M;Zhang C;Wu X;Fan T;Cao S
我们的前期研究表明MAN 3介导的甘露糖在植物对镉胁迫的响应中起着重要作用。然而，相关的潜在机制和信号通路知之甚少。在这项研究中，我们发现，拟南芥MYB 4-MAN 3-甘露糖-MNB 1信号级联参与调节植物镉耐受性。甘露糖结合凝集素1（mannose-binding-lectin 1，MNB 1）的功能缺失导致镉积累和耐性降低，而过量表达MNB 1则显著增强镉积累和耐性。与此一致，在镉胁迫下，mnb 1突变体中GSH依赖的植物螯合素（PC）合成途径相关基因（如GSH 1、GSH 2、PCS 1和PCS 2）的表达显著降低，而MNB 1-OE系中则显著增加，这与镉激活的PC合成呈正相关。此外，我们发现甘露糖能够结合到MNB 1的GNA相关结构域，并且甘露糖结合到MNB 1的GNA相关结构域是MAN 3介导的拟南芥镉耐受性所必需的。进一步的分析表明，MYB 4直接与MAN 3的启动子结合，通过GSH依赖的PC合成途径正向调节MAN 3的转录，从而调节镉的耐性。与这些发现一致，MAN 3的过表达拯救了myb 4突变体的Cd敏感表型，但不是mnb 1突变体，而MNB 1的过表达拯救了myb 4突变体的Cd敏感表型。总之，我们的研究结果提供了令人信服的证据表明，MYB 4-MAN 3-甘露糖-MNB 1信号级联调节镉耐受拟南芥通过GSH依赖的PC合成途径。
Our previous studies showed that MAN3-mediated mannose plays an important role in plant responses to cadmium (Cd) stress. However, the underlying mechanisms and signaling pathways involved are poorly understood. In this study, we showed that an Arabidopsis MYB4-MAN3-Mannose-MNB1 signaling cascade is involved in the regulation of plant Cd tolerance. Loss-of-function of MNB1 (mannose-binding-lectin 1) led to decreased Cd accumulation and tolerance, whereas overexpression of MNB1 significantly enhanced Cd accumulation and tolerance. Consistently, expression of the genes involved in the GSH-dependent phytochelatin (PC) synthesis pathway (such as GSH1, GSH2, PCS1, and PCS2) was significantly reduced in the mnb1 mutants but markedly increased in the MNB1-OE lines in the absence or presence of Cd stress, which was positively correlated with Cd-activated PC synthesis. Moreover, we found that mannose is able to bind to the GNA-related domain of MNB1, and that mannose binding to the GNA-related domain of MNB1 is required for MAN3-mediated Cd tolerance in Arabidopsis. Further analysis showed that MYB4 directly binds to the promoter of MAN3 to positively regulate the transcript of MAN3 and thus Cd tolerance via the GSH-dependent PC synthesis pathway. Consistent with these findings, overexpression of MAN3 rescued the Cd-sensitive phenotype of the myb4 mutant but not the mnb1 mutant, whereas overexpression of MNB1 rescued the Cd-sensitive phenotype of the myb4 mutant. Taken together, our results provide compelling evidence that a MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis through the GSH-dependent PC synthesis pathway.
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