Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets.
Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets.
复制标题
乙烯和ABA对淀粉合成基因表达的调控与水稻劣质和优质小穗发育的关系
DOI：
10.1093/jxb/err088
复制
发表时间：
2011-07
影响因子：
6.9
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhu G;Ye N;Yang J;Peng X;Zhang J
晚花的稻穗中的小穗，被称为下位小穗，通常是不饱满的，并且经常限制一些水稻品种的产量潜力。这种灌浆不良的生理和分子机制仍不清楚。本研究综合分析了弱势粒和上级粒淀粉合成和激素信号转导相关基因的差异表达及其与籽粒灌浆的关系。基因芯片和实时荧光定量PCR分析表明，在灌浆早中期，一组淀粉代谢相关基因在上级颖花中的表达增强，转录水平高于弱势颖花。脱落酸（阿坝）合成基因，9-顺式环氧类胡萝卜素双加氧酶1（NCED 1）和NCED 5，和乙烯合成基因，1-氨基环丙烷-1-羧酸氧化酶1（ACO 1）和ACO 3的表达，随着颖果的发育下降。同时，与弱势小穗相比，这些基因在上级小穗中的表达下降更快，转录谱也更低，尤其是乙烯。阿坝浓度和乙烯释放速率与其基因表达的变化趋势相似。外源阿坝降低了离体水稻籽粒中蔗糖合成酶（SUS）的mRNA水平及其酶活性，而外源乙烯利（乙烯释放剂）抑制了大部分淀粉合成基因SUS、ADP-葡萄糖焦磷酸化酶（AGP-glucose pyrophosphorylase，AGP-glucose pyrophosphorylase）和可溶性淀粉合成酶（soluble starch synthase，SSS）的表达，并下调了其酶活性。综上所述，弱势粒中较高浓度的乙烯和阿坝抑制了淀粉合成基因的表达及其酶活性，从而导致灌浆速率降低。
Later-flowering spikelets in a rice panicle, referred to as the inferior spikelets, are usually poorly filled and often limit the yield potential of some rice cultivars. The physiological and molecular mechanism for such poor grain filling remains unclear. In this study the differentially expressed genes in starch synthesis and hormone signalling between inferior and superior spikelets were comprehensively analysed and their relationships with grain filling was investigated. DNA microarray and real-time PCR analysis revealed that a group of starch metabolism-related genes showed enhanced expression profiles and had higher transcript levels in superior spikelets than in inferior ones at the early and middle grain-filling stages. Expression of the abscisic acid (ABA) synthesis genes, 9-cis-epoxycarotenoid dioxygenase 1 (NCED1) and NCED5, and the ethylene synthesis genes, 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) and ACO3, declined with development of the caryopses. Meanwhile, if compared with inferior spikelets, expression of these genes in superior spikelets decreased faster and had lower transcript profiles, especially for ethylene. ABA concentration and ethylene evolution rate showed similar trends to their gene expression. Exogenous supply of ABA reduced the sucrose synthase (SUS) mRNA level and its enzyme activity in detached rice grains, while exogenously supplied ethephon (an ethylene-releasing reagent) suppressed the expression of most starch synthesis genes; that is, SUS, ADP-glucose pyrophosphorylase (AGPase), and soluble starch synthase (SSS), and down-regulated their enzyme activities. In summary, it is concluded that the relatively high concentrations of ethylene and ABA in inferior spikelets suppress the expression of starch synthesis genes and their enzyme activities and consequently lead to a low grain-filling rate.
登录
查看更多内容
影响因子：
6.9
作者：
BUYSSE, J;MERCKX, R
通讯作者：
MERCKX, R
影响因子：
3
作者：
Liu, FL;Andersen, MN;Jensen, CR
通讯作者：
Jensen, CR
影响因子：
4.3
作者：
Hirose, T;Terao, T
通讯作者：
Terao, T
影响因子：
4.2
作者：
Ahmadi, A;Baker, DA
通讯作者：
Baker, DA
影响因子：
5.1
作者：
Duan, MJ;Sun, SSM
通讯作者：
Sun, SSM