Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.
Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.
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DOI：
10.1111/aji.12779
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Davies CJ
中科院分区：
文献类型：
--
作者：
Shi B;Thomas AJ;Benninghoff AD;Sessions BR;Meng Q;Parasar P;Rutigliano HM;White KL;Davies CJ
经典的主要组织相容性复合体（MHC）I类（MHC-Ia）和非经典的MHC I类（MHC-Ib）基因的差异表达的调控机制知之甚少。采用定量逆转录-聚合酶链反应（PCR）比较外周血单个核细胞（PBMC）和胎盘滋养层细胞（PTC）中MHC-I转录本和相关转录因子的丰度。通过亚硫酸氢盐处理和下一代测序检测MHC-I CpG岛的甲基化。用5′-氮脱氧胞苷对PBMC和PTC进行去甲基化，以评估甲基化在基因调控中的作用。 PBMC中的MHC-I表达高于PTC，并且与IRF 1、II类MHC反式激活因子（CIITA）和STAT 1的表达相关。MHC-Ia基因和BoLA-NC 1在PBMC和PTC中没有CpG甲基化。相比之下，BoLA-NC 2、-NC 3和-NC 4基因体中的CpG位点在PBMC中高度甲基化，但在正常PTC中大部分未甲基化，在体细胞核转移PTC中中度甲基化。在PBMC中，去甲基化导致MHC-Ib上调2.8至6倍，而MHC-Ia转录物升高不到2倍。 DNA甲基化调节牛MHC-Ib的表达，并可能导致PBMC和PTC中MHC-Ib与MHC-Ia转录物的不同相对水平。
The regulatory mechanisms governing differential expression of classical major histocompatibility complex (MHC) class I (MHC‐Ia) and non‐classical MHC class I (MHC‐Ib) genes are poorly understood. Quantitative reverse transcription‐ polymerase chain reaction (PCR) was used to compare the abundance of MHC‐I transcripts and related transcription factors in peripheral blood mononuclear cells (PBMC) and placental trophoblast cells (PTC). Methylation of MHC‐I CpG islands was detected by bisulfite treatment and next‐generation sequencing. Demethylation of PBMC and PTC with 5′‐aza‐deoxycytidine was used to assess the role of methylation in gene regulation. MHC‐I expression was higher in PBMC than PTC and was correlated with expression of IRF1, class II MHC transactivator (CIITA), and STAT1. The MHC‐Ia genes and BoLA‐NC1 were devoid of CpG methylation in PBMC and PTC. In contrast, CpG sites in the gene body of BoLA‐NC2, ‐NC3, and ‐NC4 were highly methylated in PBMC but largely unmethylated in normal PTC and moderately methylated in somatic cell nuclear transfer PTC. In PBMC, demethylation resulted in upregulation of MHC‐Ib by 2.8‐ to 6‐fold, whereas MHC‐Ia transcripts were elevated less than 2‐fold. DNA methylation regulates bovine MHC‐Ib expression and is likely responsible for the different relative levels of MHC‐Ib to MHC‐Ia transcripts in PBMC and PTC.
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影响因子：
16.8
作者：
Lorincz, MC;Dickerson, DR;Groudine, M
通讯作者：
Groudine, M
影响因子：
20.3
作者：
Agaugue, Sophie;Carosella, Edgardo D.;Rouas-Freiss, Nathalie
通讯作者：
Rouas-Freiss, Nathalie
DOI：
10.1111/j.1600-0897.2005.00364.x
发表时间：
2006-03-01
影响因子：
3.6
作者：
Davies, CJ;Eldridge, JA;Schlafer, DH
通讯作者：
Schlafer, DH
影响因子：
--
作者：
Akhter, A.;Faridi, R. M.;Agrawal, S.
通讯作者：
Agrawal, S.
影响因子：
5.4
作者：
Deaton, Aimee M.;Cook, Peter C.;De Sousa, Dina;Phythian-Adams, Alexander T.;Bird, Adrian;MacDonald, Andrew S.
通讯作者：
MacDonald, Andrew S.