Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions.
Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions.
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DOI：
10.1186/s13104-023-06432-w
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发表时间：
2023-08-04
影响因子：
1.8
通讯作者：
中科院分区：
文献类型：
--
作者：
少突胶质细胞 (OL) 是中枢神经系统中负责髓磷脂形成的神经胶质细胞类型。体外培养 OL 的能力为了解其功能背后的机制提供了重要的见解。然而，原代 OL 培养物获取繁琐、难以繁殖且不易进行遗传操作。为了克服这些障碍，研究人员培育出了来自不同物种的永生化 OL 样细胞系。其中一种细胞系是小鼠 Oli-neu 系，它被认为概括了 OL 在成熟早期阶段的特征。它们已在多项研究中广泛用作 OL 的替代物，特别是在分析 OL 谱系中的表观遗传修饰和调控途径时。在本报告中，我们介绍了优化培养基和生长条件的开发，极大地促进了 Oli-neu 细胞的分化。与之前的培养方案相比，使用这些新方案分化的 Oli-neu 细胞表现出更高的髓磷脂相关基因表达和增加的分支，这两者都是成熟 OL 的定义特征。我们预计这些新的培养条件将极大地促进 Oli-neu 细胞的使用，并增强其重现原代 OL 显着特征的能力。在线版本包含可在 10.1186/s13104-023-06432-w 获取的补充材料。
Oligodendrocytes (OL) are the glial cell type in the CNS that are responsible for myelin formation. The ability to culture OLs in vitro has provided critical insights into the mechanisms underlying their function. However, primary OL cultures are tedious to obtain, difficult to propagate and are not easily conducive to genetic manipulation. To overcome these obstacles, researchers have generated immortalized OL like cell lines derived from various species. One such cell line is the mouse Oli-neu line which is thought to recapitulate characteristics of OLs in early stages of maturity. They have been extensively utilized in multiple studies as surrogates for OLs, especially in analyzing epigenetic modifications and regulatory pathways in the OL lineage. In this report we present the development of optimized culture media and growth conditions that greatly facilitate the differentiation of Oli-neu cells. Oli-neu cells differentiated using these new protocols exhibit a higher expression of myelin related genes and increased branching, both of which are defining characteristics of mature OLs, when compared to previous culture protocols. We envision that these new culture conditions will greatly facilitate the use of Oli-neu cells and enhance their ability to recapitulate the salient features of primary OLs. The online version contains supplementary material available at 10.1186/s13104-023-06432-w.
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影响因子：
3.1
作者：
Barateiro, Andreia;Brites, Dora;Fernandes, Adelaide
通讯作者：
Fernandes, Adelaide
影响因子：
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Vanderver, Adeline
影响因子：
4.3
作者：
Hyrien, O;Mayer-Pröschel, M;Yakovlev, A
通讯作者：
Yakovlev, A
影响因子：
4.4
作者：
Krueger, WHH;Madison, DL;Pfeiffer, SE
通讯作者：
Pfeiffer, SE
影响因子：
14.5
作者：
do Rosario, Michelle C.;Bey, Guillermo Rodriguez;Shukla, Anju
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Shukla, Anju