Transcriptome profiling of sexual maturation and mating in the Mediterranean fruit fly, Ceratitis capitata.
Transcriptome profiling of sexual maturation and mating in the Mediterranean fruit fly, Ceratitis capitata.
复制标题
DOI：
10.1371/journal.pone.0030857
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gasperi G
中科院分区：
文献类型：
--
作者：
Gomulski LM;Dimopoulos G;Xi Z;Scolari F;Gabrieli P;Siciliano P;Clarke AR;Malacrida AR;Gasperi G
昆虫的性成熟和交配通常伴随着重大的生理和行为变化。这些变化中的许多都与寻找配偶的需要有关，然后，就雌性而言，从寻找配偶转向产卵行为。地中海果蝇（Ceratitis capitata）惊人的繁殖能力是其成功成为入侵害虫物种的因素之一。为了确定与雄性和雌性蝇成熟和交配状态相关的分子变化，采用基于微阵列的基因表达方法比较了性未成熟、成熟处女和交配个体的头部转录组。注意力集中在与生殖、行为、化学刺激的感觉知觉和免疫系统过程相关的转录本丰度的变化上。在雌性成熟过程中记录了广泛的转录变化，而相比之下，雌性交配后的转录变化则不大。在雄性蝶蝇中，转录变化在成熟和交配过程中都是一致的。特别值得注意的是，在黑腹果蝇中没有记录到的交配诱导的免疫反应，这可能是由于这些物种的生殖策略不同。这项研究，除了增加我们对medfly成熟和交配背后的分子机制的理解之外，还确定了重要的基因靶点，可能对未来管理这种害虫有用。
Sexual maturation and mating in insects are generally accompanied by major physiological and behavioural changes. Many of these changes are related to the need to locate a mate and subsequently, in the case of females, to switch from mate searching to oviposition behaviour. The prodigious reproductive capacity of the Mediterranean fruit fly, Ceratitis capitata, is one of the factors that has led to its success as an invasive pest species. To identify the molecular changes related to maturation and mating status in male and female medfly, a microarray-based gene expression approach was used to compare the head transcriptomes of sexually immature, mature virgin, and mated individuals. Attention was focused on the changes in abundance of transcripts related to reproduction, behaviour, sensory perception of chemical stimulus, and immune system processes. Broad transcriptional changes were recorded during female maturation, while post-mating transcriptional changes in females were, by contrast, modest. In male medfly, transcriptional changes were consistent both during maturation and as a consequence of mating. Of particular note was the lack of the mating-induced immune responses that have been recorded for Drosophila melanogaster, that may be due to the different reproductive strategies of these species. This study, in addition to increasing our understanding of the molecular machinery behind maturation and mating in the medfly, has identified important gene targets that might be useful in the future management of this pest.
登录
查看更多内容
影响因子：
4.4
作者：
Ellis LL;Carney GE
通讯作者：
Carney GE
影响因子：
--
作者：
Bebas P;Kotwica J;Joachimiak E;Giebultowicz JM
通讯作者：
Giebultowicz JM
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
30.8
作者：
Anholt, RRH;Dilda, CL;Mackay, TFC
通讯作者：
Mackay, TFC
影响因子：
1.9
作者：
Diamantidis, Alexandros D.;Papadopoulos, Nikos T.;Carey, James R.
通讯作者：
Carey, James R.