Glia co-culture with neurons in microfluidic platforms promotes the formation and stabilization of synaptic contacts.
Glia co-culture with neurons in microfluidic platforms promotes the formation and stabilization of synaptic contacts.
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DOI：
10.1039/c3lc50249j
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发表时间：
2013-08-07
期刊：
影响因子：
6.1
通讯作者：
Webb DJ
中科院分区：
文献类型：
--
作者：
Shi M;Majumdar D;Gao Y;Brewer BM;Goodwin CR;McLean JA;Li D;Webb DJ
开发了两种新的微流控细胞培养方案，垂直分层和四室结构，用于中枢神经系统(CNS)神经元和神经胶质细胞的联合培养。这些设备中的细胞室由压力驱动的阀门屏障隔开，这允许我们控制两种细胞类型之间的通信。这些装置的独特设计促进了神经胶质细胞与邻近神经元(~50-100μm)的共培养，神经元群体的差异转染，以及神经元相互作用的动态可视化，如突触的发育。利用这些共培养装置，用高分辨率荧光显微镜观察不同荧光标记物(如GFP和mCherry-Synaptophysin)转染的神经元之间的初始突触接触。神经胶质细胞的存在通过增加突触接触的数量和稳定性对突触产生了深远的影响。有趣的是，通过液相色谱-离子迁移率-质谱仪的检测，神经元-神经胶质细胞共培养产生的可溶性因子水平高于单独的神经元或神经胶质细胞培养物，这表明了神经元-胶质细胞相互作用调节突触功能的潜在机制。综上所述，这些结果表明神经元和胶质细胞之间的通信对于突触的形成和稳定性是至关重要的，并指出了发展神经元-胶质细胞共培养系统的重要性，如本研究中描述的微流体平台。
Two novel microfluidic cell culture schemes, a vertically-layered set-up and a four chamber set-up, were developed for co-culturing central nervous system (CNS) neurons and glia. The cell chambers in these devices were separated by pressure-enabled valve barriers, which permitted us to control communication between the two cell types. The unique design of these devices facilitated the co-culture of glia with neurons in close proximity (~50–100 μm), differential transfection of neuronal populations, and dynamic visualization of neuronal interactions, such as the development of synapses. With these co-culture devices, initial synaptic contact between neurons transfected with different fluorescent markers, such as GFP and mCherry-synaptophysin, was imaged using high-resolution fluorescence microscopy. The presence of glial cells had a profound influence on synapses by increasing the number and stability of synaptic contacts. Interestingly, as determined by liquid chromatography-ion mobility-mass spectrometry, neuron-glia co-cultures produced elevated levels of soluble factors compared to that secreted by individual neuron or glia cultures, suggesting a potential mechanism by which neuron-glia interactions could modulate synaptic function. Collectively, these results show that communication between neurons and glia is critical for the formation and stability of synapses and point to the importance of developing neuron-glia co-culture systems such as the microfluidic platforms described in this study.
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DOI：
10.1523/jneurosci.0203-12.2012
发表时间：
2012-05-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hosmane S;Tegenge MA;Rajbhandari L;Uapinyoying P;Ganesh Kumar N;Thakor N;Venkatesan A
通讯作者：
Venkatesan A
影响因子：
5.7
作者：
Chen JL;Nedivi E
通讯作者：
Nedivi E
影响因子：
6.1
作者：
Hosmane, Suneil;Yang, In Hong;Venkatesan, Arun
通讯作者：
Venkatesan, Arun
影响因子：
3.9
作者：
Roman, GT;Culbertson, CT
通讯作者：
Culbertson, CT
通过神经胶质调节突触连通性。
影响因子：
64.8
作者：
Eroglu, Cagla;Barres, Ben A.
通讯作者：
Barres, Ben A.