Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses.
Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses.
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DOI：
10.1038/srep04680
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发表时间：
2014-04-15
影响因子：
4.6
通讯作者：
Akaike T
中科院分区：
文献类型：
--
作者：
Khan S;Rahman HN;Okamoto T;Matsunaga T;Fujiwara Y;Sawa T;Yoshitake J;Ono K;Ahmed KA;Rahaman MM;Oyama K;Takeya M;Ida T;Kawamura Y;Fujii S;Akaike T
同性恋螺杆菌是在人类中引起菌血症的最常见的肝肠螺杆菌种类，但其致病性尚不清楚。在此，我们在体内和体外研究了同性恋螺杆菌与动脉粥样硬化可能存在的关联。我们发现同性恋螺杆菌感染显著加剧了高脂血症小鼠的动脉粥样硬化。感染小鼠的主动脉根部病变显示中性粒细胞和F4/80⁺泡沫细胞的积聚增加，这至少部分是由于细菌介导的促炎基因表达增加所致。尽管感染无症状，但检测到同性恋螺杆菌的细胞致死膨胀毒素RNA表明主动脉受到感染。同性恋螺杆菌感染改变了培养的巨噬细胞中胆固醇受体和转运蛋白的表达，并导致泡沫细胞形成。此外，感染诱导了THP - 1单核细胞的分化。这些数据首次提供了同性恋螺杆菌在实验模型中对动脉粥样硬化具有致病作用的证据，从而为进一步研究肝肠螺杆菌属在动脉粥样硬化和心血管疾病中可能的作用提供了依据。
Helicobacter cinaedi is the most common enterohepatic Helicobacter species that causes bacteremia in humans, but its pathogenicity is unclear. Here, we investigated the possible association of H. cinaedi with atherosclerosis in vivo and in vitro. We found that H. cinaedi infection significantly enhanced atherosclerosis in hyperlipidaemic mice. Aortic root lesions in infected mice showed increased accumulation of neutrophils and F4/80+ foam cells, which was due, at least partly, to bacteria-mediated increased expression of proinflammatory genes. Although infection was asymptomatic, detection of cytolethal distending toxin RNA of H. cinaedi indicated aorta infection. H. cinaedi infection altered expression of cholesterol receptors and transporters in cultured macrophages and caused foam cell formation. Also, infection induced differentiation of THP-1 monocytes. These data provide the first evidence of a pathogenic role of H. cinaedi in atherosclerosis in experimental models, thereby justifying additional investigations of the possible role of enterohepatic Helicobacter spp. in atherosclerosis and cardiovascular disease.
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影响因子：
4.9
作者：
Linton, MF;Fazio, S
通讯作者：
Fazio, S
影响因子：
5.3
作者：
Brenner, H;Berg, G;Koenig, W
通讯作者：
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Hsich, E;Zhou, YF;Epstein, SE
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作者：
Li, Li;Thompson, Patricia A.;Kitchens, Richard L.
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Kitchens, Richard L.
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9.4
作者：
Fernandez, KR;Hansen, LM;Solnick, JV
通讯作者：
Solnick, JV