Switchable Photoacoustic Intensity of Methylene Blue via Sodium Dodecyl Sulfate Micellization.
Switchable Photoacoustic Intensity of Methylene Blue via Sodium Dodecyl Sulfate Micellization.
复制标题
DOI：
10.1021/acs.langmuir.7b03718
复制
发表时间：
2018-01-09
期刊：
影响因子：
--
通讯作者：
Jokerst JV
中科院分区：
文献类型：
--
作者：
Wang J;Lin CY;Moore C;Jhunjhunwala A;Jokerst JV
亚甲基蓝（MB）与十二烷基硫酸钠（SDS）之间的相互作用已被广泛研究，但其相互作用的细节尚不清楚。在这里，我们将光声（PA）成像与纳米颗粒跟踪分析（NTA）和光谱学结合起来进一步阐明这种相互作用。加入3.47 mM SDS后，0.05 mM MB的PA成像显示强度增加492倍。SDS临界胶束浓度（CMC）为8.67 mM以上，浓度越高，PA强度降低54倍。相对量子产率测量表明，由于荧光猝灭，PA强度增加。同时，NTA表明，SDS浓度低于CMC时，大小在80 ~ 400 nm之间的非胶束MB/SDS团簇数量增加，高于CMC时则减少。这一趋势表明MB/SDS簇对PA强度的增强负有责任。与MB/十六烷基三甲基溴化铵、MB/硫酸辛基钠和MB/氯化钠的光谱位移和光谱强度的比较表明，MB在胶束前后与SDS的硫酸盐部分结合。我们的观察表明，在胶束浓度下，MB与SDS形成聚集体，并且MB聚集体作为单体分离，与胶束浓度下的SDS的硫酸盐部分结合。这些发现进一步阐明了MB和SDS相互作用的过程，并展示了开发基于MB /SDS的造影剂的潜力。
The interaction between methylene blue (MB) and sodium dodecyl sulfate (SDS) has been widely studied spectroscopically, but details about their interactions remain unclear. Here, we combined photoacoustic (PA) imaging with nanoparticle tracking analysis (NTA) and spectroscopy to further elucidate this interaction. PA imaging of 0.05 mM MB showed a 492-fold increase in intensity upon the addition of 3.47 mM SDS. Higher concentrations above SDS’s critical micelle concentration (CMC) at 8.67 mM decreased the PA intensity by 54 times. Relative quantum yield measurements indicated that PA intensity increased as a result of fluorescence quenching. Meanwhile, NTA indicated an increased number of nonmicellar MB/SDS clusters at SDS concentrations below the CMC varying in size from 80 to 400 nm as well as a decreased number above the CMC. This trend suggested that MB/SDS clusters are responsible for the PA intensity enhancement. Comparison of PA intensities and spectral shifts with MB/hexadecyltrimethylammonium bromide, MB/sodium octyl sulfate, and MB/sodium chloride demonstrated that MB was bound to the sulfate moiety of SDS before and after micellization. Our observations suggest that MB forms aggregates with SDS at premicellar concentrations, and the MB aggregates disassociate as monomers that are bound to the sulfate moiety of SDS at micellar concentrations. These findings further clarify the process by which MB and SDS interact and demonstrate the potential for developing MB-/SDS-based contrast agents.
登录
查看更多内容
影响因子：
3.5
作者：
Ermilov, Sergey A.;Khamapirad, Tuenchit;Oraevsky, Alexander A.
通讯作者：
Oraevsky, Alexander A.
影响因子：
3.5
作者：
Ashkenazi, Shai
通讯作者：
Ashkenazi, Shai
影响因子：
3.3
作者：
Dean, Jacob C.;Oblinsky, Daniel G.;Scholes, Gregory D.
通讯作者：
Scholes, Gregory D.
影响因子：
--
作者：
BRASWELL, E
通讯作者：
BRASWELL, E
影响因子：
3.5
作者：
Hariri, Ali;Fatima, Afreen;Avanaki, Mohammad R. N.
通讯作者：
Avanaki, Mohammad R. N.