ASPM Is a Prognostic Biomarker and Correlates With Immune Infiltration in Kidney Renal Clear Cell Carcinoma and Liver Hepatocellular Carcinoma.
ASPM Is a Prognostic Biomarker and Correlates With Immune Infiltration in Kidney Renal Clear Cell Carcinoma and Liver Hepatocellular Carcinoma.
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DOI：
10.3389/fonc.2022.632042
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发表时间：
2022
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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异常纺锤体微管组装（ASPM）是一种中心体蛋白，与不良的临床预后和复发有关。然而，ASPM表达、肿瘤免疫和不同癌症预后之间的关系仍不清楚。使用肿瘤免疫评估资源（TIMER）、UALCAN、OncoLnc和基因表达谱交互分析（GEPIA）数据库分析ASPM表达及其对肿瘤预后的影响。使用TIMER和GEPIA数据库分析ASPM表达与肿瘤免疫的关系，并使用qPCR、western blot和多重定量免疫荧光进一步验证结果。结果显示，ASPM在肾透明细胞癌（KIRC）、肾乳头状细胞癌（KIRP）、肝细胞癌（LIHC）、肺腺癌（LUAD）、胰腺癌（PAAD）和乳腺浸润癌（BRCA）中的表达明显高于相应的正常组织。晚期癌症中ASPM表达显著高于早期癌症（例如，KIRC、KIRP、LIHC、LUAD和BRCA; p < 0.05），证明ASPM在癌症进展和侵袭中的可能作用。此外，我们的数据显示，高ASPM表达与KIRC和LIHC的总生存率和疾病特异性生存率相关（p < 0.05）。此外，考克斯风险回归分析结果显示，ASPM可能是KIRC和LIHC的独立预后因素。ASPM表达与KIRC和LIHC中的肿瘤浸润B细胞、CD 8 + T细胞和M2巨噬细胞密切相关。这些发现表明，ASPM的高表达表明预后不良以及KIRC和LIHC中免疫细胞浸润水平的增加。ASPM表达可作为KIRC和LIHC中临床结果和免疫细胞浸润的新型预后生物标志物。
Abnormal spindle microtubule assembly (ASPM) is a centrosomal protein and that is related to a poor clinical prognosis and recurrence. However, the relationship between ASPM expression, tumor immunity, and the prognosis of different cancers remains unclear. ASPM expression and its influence on tumor prognosis were analyzed using the Tumor Immune Estimation Resource (TIMER), UALCAN, OncoLnc, and Gene Expression Profiling Interactive Analysis (GEPIA) databases. The relationship between ASPM expression and tumor immunity was analyzed using the TIMER and GEPIA databases, and the results were further verified using qPCR, western blot, and multiplex quantitative immuno fluorescence. The results showed that ASPM expression was significantly higher in most cancer tissues than in corresponding normal tissues, including kidney renal clear cell carcinoma (KIRC), kidney renal papillary cell carcinoma (KIRP), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), pancreatic adenocarcinoma (PAAD), and breast invasive carcinoma (BRCA). ASPM expression was significantly higher in late-stage cancers than in early-stages cancers (e.g., KIRC, KIRP, LIHC, LUAD, and BRCA; p < 0.05), demonstrating a possible role of ASPM in cancer progression and invasion. Moreover, our data showed that high ASPM expression was associated with poor overall survival, and disease-specific survival in KIRC and LIHC (p < 0.05). Besides, Cox hazard regression analysis results showed that ASPM may be an independent prognostic factor for KIRC and LIHC. ASPM expression showed a strong correlation with tumor-infiltrating B cells, CD8+ T cells, and M2 macrophages in KIRC and LIHC. These findings demonstrate that the high expression of ASPM indicates poor prognosis as well as increased levels of immune cell infiltration in KIRC and LIHC. ASPM expression may serve as a novel prognostic biomarker for both the clinical outcome and immune cell infiltration in KIRC and LIHC.
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