Effects of orthopedic polymer particles on chemotaxis of macrophages and mesenchymal stem cells.
Effects of orthopedic polymer particles on chemotaxis of macrophages and mesenchymal stem cells.
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DOI：
10.1002/jbm.a.32803
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Goodman, Stuart B.
中科院分区：
文献类型：
--
作者：
Huang, Zhinong;Ma, Ting;Ren, Pei-Gen;Smith, R. Lane;Goodman, Stuart B.
全关节置换术（TJA）产生的磨损颗粒刺激巨噬细胞释放趋化因子。尚未阐明从磨损颗粒刺激的巨噬细胞释放的趋化因子对体外巨噬细胞和骨祖细胞迁移的作用。在本研究中，我们用临床相关的聚甲基丙烯酸甲酯（PMMA，1-10 μm）和超高分子量聚乙烯（UHMWPE，2-3 μm）颗粒激发小鼠巨噬细胞（RAW 264.7）。以人巨噬细胞（THP-1）和人骨髓间充质干细胞（MSC）为迁移细胞，体外检测条件培养基（CM）的趋化作用。从两种颗粒类型收集的CM对人巨噬细胞具有趋化作用，其可被单核细胞趋化蛋白-1（MCP-1）中和抗体消除。阻断CCR 1受体消除了趋化作用，而CCR 2抗体仅部分降低THP-1细胞迁移。来自PMMA而不是CPE暴露的巨噬细胞的CM导致MSC的趋化性;这种作用可以被巨噬细胞炎性蛋白-1 α（MIP-1α）中和抗体消除。CCR 1和CCR 2阻断抗体均未显示对MSC迁移的影响。由磨损颗粒刺激的巨噬细胞释放的趋化因子可以对巨噬细胞和MSC的迁移产生影响。这种作用似乎依赖于颗粒类型，并且可能受到MCP-1和MIP-1α的调节，然而，趋化性可能需要不止一种趋化因子。
Wear particles generated from total joint arthroplasty (TJA) stimulate macrophages to release chemokines. The role of chemokines released from wear particle-stimulated macrophages on the migration of macrophages and osteoprogenitor cells in vitro has not been elucidated. In this study, we challenged murine macrophages (RAW 264.7) with clinically relevant polymethyl methacrylate (PMMA, 1-10 μm) and ultra high molecular weight polyethylene (UHMWPE, 2-3 μm) particles. The chemotactic effects of the conditioned media (CM) were tested in vitro using human macrophages (THP-1) and human mesenchymal stem cells (MSCs) as the migrating cells. CM collected from both particle types had a chemotactic effect on human macrophages, which could be eliminated by monocyte chemotactic protein-1 (MCP-1) neutralizing antibody. Blocking the CCR1 receptor eliminated the chemotactic effect, while CCR2 antibody only partially decreased THP-1 cell migration. CM from PMMA but not UHMWPE-exposed macrophages led to chemotaxis of MSCs; this effect could be eliminated by macrophage inflammatory protein-1 alpha (MIP-1α) neutralizing antibody. Neither CCR1 nor CCR2 blocking antibodies showed an effect on the migration of MSCs. Chemokines released by macrophages stimulated by wear particles can have an effect on the migration of macrophages and MSCs. This effect seems to be dependent on the particle type, and may be modulated by MCP-1 and MIP-1α, however more than one chemokine may be necessary for chemotaxis.
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DOI：
10.1097/00003086-199707000-00016
发表时间：
1997-07-01
影响因子：
4.2
作者：
Kadoya, Y;Revell, PA;Freeman, MAR
通讯作者：
Freeman, MAR
DOI：
10.2106/00004623-199509000-00002
发表时间：
1995-09-01
影响因子：
5.3
作者：
MALONEY, WJ;SMITH, RL;RUBASH, H
通讯作者：
RUBASH, H
DOI：
10.1302/0301-620x.81b1.8884
发表时间：
1999-01-01
影响因子：
--
作者：
Nakashima, Y;Sun, DH;Smith, RL
通讯作者：
Smith, RL
DOI：
10.5435/00124635-200800001-00013
发表时间：
2008-01-01
影响因子：
3.2
作者：
Bostrom, Mathias;O'Keefe, Regis
通讯作者：
O'Keefe, Regis
DOI：
10.1243/0954411971534304
发表时间：
1997-01-01
影响因子：
1.8
作者：
Revell, PA;ALSaffar, N;Kobayashi, A
通讯作者：
Kobayashi, A