Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.
Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.
复制标题
棉花中的钙依赖性蛋白激酶：深入了解植物对盐胁迫的早期反应
DOI：
10.1186/s12870-018-1230-8
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发表时间：
2018-01-17
影响因子：
5.3
通讯作者：
Song CP
中科院分区：
文献类型：
--
作者：
Gao W;Xu FC;Guo DD;Zhao JR;Liu J;Guo YW;Singh PK;Ma XN;Long L;Botella JR;Song CP
背景土壤盐碱化是制约全球植物生长和农业生产的主要环境因素之一。涉及钙离子的信号成分(Ca~(2+))及其下游的钙依赖蛋白激酶(CPK)在胁迫信号的感知和转导中起着关键作用。结果从陆地棉(Gossypium hirsuumL.)中鉴定出98个预测的CPK。‘TM-1’)，系统发育分析将其分为4个类群。基因家族分布研究揭示了基因组复制事件对GhCPK总数的重要影响。转录组分析表明，CPKs在不同器官中的表达广泛分布。共有19个CPK在转录水平上对盐胁迫做出了快速反应，其中大部分也是由乙烯释放化学物质乙烯利诱导的，表明盐和乙烯反应的部分重叠。其中4个CPK基因(GhCPK8、GhCPK38、GhCPK54和GhCPK55)的沉默严重削弱了棉花对盐胁迫的基础抗性。结论我们对陆地棉CPK基因的全基因组表达分析表明，CPK参与了多种发育反应以及对不同非生物胁迫的反应。一组棉花CPK参与了棉花对盐胁迫反应的早期信号事件。我们的结果为棉花CPK的功能分析提供了重要的启示，特别是在棉花适应盐胁迫的背景下。
BackgroundSoil salinization is one of the major environmental constraints to plant growth and agricultural production worldwide. Signaling components involving calcium (Ca2+) and the downstream calcium-dependent protein kinases (CPKs) play key roles in the perception and transduction of stress signals. However, the study of CPKs in cotton and their functions in response to salt stress remain unexplored.ResultsA total of 98 predicted CPKs were identified from upland cotton (Gossypium hirsutumL. ‘TM-1’), and phylogenetic analyses classified them into four groups. Gene family distribution studies have revealed the substantial impacts of the genome duplication events to the total number ofGhCPKs. Transcriptome analyses showed a wide distribution ofCPKs’ expression among different organs. A total of 19CPKs were selected for their rapid responses to salt stress at the transcriptional level, most of which were also incduced by the thylene-releasing chemical ethephon, suggesting a partal overlap of the salinity and ethylene responses. Silencing of 4 of the 19CPKs(GhCPK8,GhCPK38,GhCPK54, andGhCPK55) severely compromised the basal cotton resistance to salt stress.ConclusionsOur genome-wide expression analysis ofCPKgenes from up-land cotton suggests thatCPKs are involved in multiple developmental responses as well as the response to different abiotic stresses. A cluster of the cottonCPKs was shown to participate in the early signaling events in cotton responses to salt stress. Our results provide significant insights on functional analysis ofCPKs in cotton, especially in the context of cotton adaptions to salt stress.
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