Molecular characterization of rotavirus strains detected during a clinical trial of a human rotavirus vaccine in Blantyre, Malawi.
Molecular characterization of rotavirus strains detected during a clinical trial of a human rotavirus vaccine in Blantyre, Malawi.
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DOI：
10.1016/j.vaccine.2011.09.119
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发表时间：
2012-04-27
期刊：
影响因子：
5.5
通讯作者：
Cunliffe, Nigel A.
中科院分区：
文献类型：
--
作者：
Nakagomi, Toyoko;Nakagomi, Osamu;Dove, Winifred;Yen Hai Doan;Witte, Desiree;Ngwira, Bagrey;Todd, Stacy;Steele, A. Duncan;Neuzil, Kathleen M.;Cunliffe, Nigel A.
在南非和马拉维的一项临床试验中，人类G1P[8]轮状病毒疫苗（Rotarix）显著减少了严重轮状病毒胃肠炎的发作，但马拉维的疫苗效力（49.5%）低于南非（76.9%）和其他地方报道的疫苗效力。本研究的目的是检查在马拉维临床试验期间检测到的循环野生型轮状病毒与RIX4414 （Rotarix中含有的毒株）和常见人类轮状病毒毒株的分子关系。在88例轮状病毒阳性的腹泻粪便标本中，聚丙烯酰胺凝胶电泳检查时，43例轮状病毒表现出可识别的RNA迁移模式。对基因型为G12P[6]、G1P[8]、G9P[8]和G8P[4]的5株具有代表性的菌株的VP7、VP4、VP6和NSP4基因进行了测序。它们的VP7 (G)和VP4 (P)基因型被证实，VP6 (I)和NSP4 (E)基因型为I1E1或I2E2，表明它们起源于人类轮状病毒。使用21个适应培养的菌株进行RNA-RNA杂交表明，马拉维轮状病毒具有与wa样或DS-1样人类轮状病毒共同的基因组RNA群。总体而言，马拉维毒株的基因组成似乎与疫苗效力较高的国家描述的轮状病毒相似，这表明马拉维的较低效力不太可能用流行毒株的多样性来解释。
The human, G1P[8] rotavirus vaccine (Rotarix) significantly reduced severe rotavirus gastroenteritis episodes in a clinical trial in South Africa and Malawi, but vaccine efficacy was lower in Malawi (49.5%) than reported in South Africa (76.9%) and elsewhere. The aim of this study was to examine the molecular relationships of circulating wild-type rotaviruses detected during the clinical trial in Malawi to RIX4414 (the strain contained in Rotarix) and to common human rotavirus strains. Of 88 rotavirus-positive, diarrhoeal stool specimens, 43 rotaviruses exhibited identifiable RNA migration patterns when examined by polyacrylamide gel electrophoresis. The genes encoding VP7, VP4, VP6 and NSP4 of 5 representative strains possessing genotypes G12P[6], G1P[8], G9P[8], and G8P[4] were sequenced. While their VP7 (G) and VP4 (P) genotype designations were confirmed, the VP6 (I) and NSP4 (E) genotypes were either I1E1 or I2E2, indicating that they were of human rotavirus origin. RNA-RNA hybridization using 21 culture-adapted strains showed that Malawian rotaviruses had a genomic RNA constellation common to either the Wa-like or DS-1 like human rotaviruses. Overall, the Malawi strains appear similar in their genetic make-up to rotaviruses described in countries where vaccine efficacy is greater, suggesting that the lower efficacy in Malawi is unlikely to be explained by the diversity of circulating strains.
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影响因子：
5.4
作者：
Matthijnssens, Jelle;Ciarlet, Max;Van Ranst, Marc
通讯作者：
Van Ranst, Marc
影响因子：
2.7
作者：
NAKAGOMI, O;NAKAGOMI, T
通讯作者：
NAKAGOMI, T
使用所有11个基因组RNA片段的轮状病毒组分类的建议。
影响因子：
2.7
作者：
Matthijnssens, Jelle;Ciarlet, Max;Rahman, Mustafizur;Attoui, Houssam;Banyai, Krisztian;Estes, Mary K.;Gentsch, Jon R.;Iturriza-Gomara, Miren;Kirkwood, Carl D.;Martella, Vito;Mertens, Peter P. C.;Nakagomi, Osamu;Patton, John T.;Ruggeri, Franco M.;Saif, Linda J.;Santos, Norma;Steyer, Andrej;Taniguchi, Koki;Desselberger, Ulrich;Van Ranst, Marc
通讯作者：
Van Ranst, Marc
影响因子：
9.4
作者：
GOUVEA, V;GLASS, RI;FANG, ZY
通讯作者：
FANG, ZY
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M