Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912.
Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912.
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DOI：
10.1038/s41598-020-77402-6
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发表时间：
2020-11-26
影响因子：
4.6
通讯作者：
Farnham G
中科院分区：
文献类型：
--
作者：
Llewellyn CA;Greig C;Silkina A;Kultschar B;Hitchings MD;Farnham G
“紫外线防晒”化合物，即类菌孢素氨基酸（MAA）在蓝藻中被广泛报道，并且已知在紫外线（UV）光下被诱导。然而，远红光（FR）对MAA生物合成的影响尚未研究。我们报告的结果，从两个实验测量转录调控的MAA和芳香族氨基酸途径的丝状蓝藻Chlorogloeopsis fritschii PCC 6912。第一个实验，比较紫外线与白色光，显示了特征性MAA mys基因簇的预期上调。第二个实验，将FR与白色光进行比较，显示了在FR光下，编码至多至霉孢菌素-甘氨酸的四个mys基因簇中的三个基因也被上调。这是一个新发现。我们使用HPLC分析观察到在FR光下MAA相应增加。色氨酸途径在UV下上调，在FR下无变化。酪氨酸和苯丙氨酸途径在两种条件下均未改变。然而，硝酸盐ABC转运蛋白基因上调UV和FR光下，这两种光条件下的氮需求增加。在FR光下MAAs上调的发现支持MAAs在光子耗散和温度调节中发挥作用，可能在促进地球表面温度调节中发挥作用。
The “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
16.6
作者：
Couradeau E;Karaoz U;Lim HC;Nunes da Rocha U;Northen T;Brodie E;Garcia-Pichel F
通讯作者：
Garcia-Pichel F
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.3
作者：
LIDDELL, PA;NEMETH, GA;GUST, D
通讯作者：
GUST, D
影响因子：
3.2
作者：
Huang, LX;McCluskey, MP;LaRossa, RA
通讯作者：
LaRossa, RA