Particle length-dependent titanium dioxide nanomaterials toxicity and bioactivity.
Particle length-dependent titanium dioxide nanomaterials toxicity and bioactivity.
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DOI：
10.1186/1743-8977-6-35
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发表时间：
2009-12-31
影响因子：
10
通讯作者：
Holian A
中科院分区：
文献类型：
--
作者：
Hamilton RF;Wu N;Porter D;Buford M;Wolfarth M;Holian A
二氧化钛(Ti02)纳米材料在光催化剂和太阳能电池方面有相当大的有利用途。多年来，人们已经确定，颜料级二氧化钛(200纳米球)在体内或体外进入生物模型系统时是相对惰性的。因此，在生物暴露的应用中，二氧化钛纳米材料被认为是一种有吸引力的替代材料。不幸的是，纳米尺度上的金属氧化物(一维100纳米)可能会也可能不会表现出与原始材料相同的毒性潜力。另一个更复杂的问题是对纳米材料进行修饰或工程，使其在结构和几何上与原始材料不同。合成了短(<；5μm)和长(>；15μm)纳米带，用原代小鼠肺泡巨噬细胞和小鼠体内实验对其进行了表征和生物活性测试。这项研究表明，锐钛矿型二氧化钛纳米材料转变为大于15μm的纤维结构会产生剧毒颗粒，并引发肺泡巨噬细胞的炎症反应。这些纤维状纳米材料通过组织蛋白酶B介导的机制诱导炎性小体激活和炎性细胞因子的释放。因此，长的二氧化钛纳米带以非常类似于石棉或二氧化硅的方式与肺巨噬细胞相互作用。这些观察表明，对纳米材料的任何修饰，导致金属丝、纤维、皮带或管子，都需要测试其致病潜力。正如这项研究所表明的那样，随着材料的形状改变为吞噬细胞难以处理的形状，毒性和致病潜力发生了戏剧性的变化，导致溶酶体的破坏。
Titanium dioxide (TiO2) nanomaterials have considerable beneficial uses as photocatalysts and solar cells. It has been established for many years that pigment-grade TiO2 (200 nm sphere) is relatively inert when internalized into a biological model system (in vivo or in vitro). For this reason, TiO2 nanomaterials are considered an attractive alternative in applications where biological exposures will occur. Unfortunately, metal oxides on the nanoscale (one dimension < 100 nm) may or may not exhibit the same toxic potential as the original material. A further complicating issue is the effect of modifying or engineering of the nanomaterial to be structurally and geometrically different from the original material. TiO2 nanospheres, short (< 5 μm) and long (> 15 μm) nanobelts were synthesized, characterized and tested for biological activity using primary murine alveolar macrophages and in vivo in mice. This study demonstrates that alteration of anatase TiO2 nanomaterial into a fibre structure of greater than 15 μm creates a highly toxic particle and initiates an inflammatory response by alveolar macrophages. These fibre-shaped nanomaterials induced inflammasome activation and release of inflammatory cytokines through a cathepsin B-mediated mechanism. Consequently, long TiO2 nanobelts interact with lung macrophages in a manner very similar to asbestos or silica. These observations suggest that any modification of a nanomaterial, resulting in a wire, fibre, belt or tube, be tested for pathogenic potential. As this study demonstrates, toxicity and pathogenic potential change dramatically as the shape of the material is altered into one that a phagocytic cell has difficulty processing, resulting in lysosomal disruption.
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影响因子：
4.5
作者：
Gurr, JR;Wang, ASS;Jan, KY
通讯作者：
Jan, KY
影响因子：
4
作者：
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通讯作者：
Lewis, James P.
影响因子：
4.4
作者：
Pelegrin, Pablo;Barroso-Gutierrez, Consuelo;Surprenant, Annmarie
通讯作者：
Surprenant, Annmarie
影响因子：
30.5
作者：
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影响因子：
56.9
作者：
Dostert, Catherine;Petrilli, Virginie;Tschopp, Jurg
通讯作者：
Tschopp, Jurg