Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome.
Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome.
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DOI：
10.3109/17435390.2015.1078854
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发表时间：
2016
期刊：
影响因子：
5
通讯作者：
Bergin IL
中科院分区：
文献类型：
--
作者：
Wilding LA;Bassis CM;Walacavage K;Hashway S;Leroueil PR;Morishita M;Maynard AD;Philbert MA;Bergin IL
银纳米颗粒 (AgNP) 已在多种应用中用作抗菌剂，包括局部伤口敷料以及消费产品和生物医学设备的涂层。摄入是银纳米颗粒暴露的一个相关途径，无论是通过消费产品中的银溶解无意发生的，还是通过膳食补充剂有意摄入的。 AgNP 也被提议作为动物饲料中抗生素的替代品。虽然已知口服抗生素对肠道细菌有显着影响，但摄入的纳米银颗粒对本土微生物组或肠道病原体的抗菌作用尚不清楚。此外，AgNP 的尺寸和涂层被认为对其生化特性有显着影响，但这些特性对抗菌功效的影响尚不清楚。在重复口服两种不同尺寸（20 和 110 nm）和涂层（PVP 和柠檬酸盐）的特征明确的 AgNP 28 天后，我们使用 16S rRNA 基因片段的培养独立测序来评估小鼠肠道微生物群落。无论大小或涂层如何，每天口服 10 毫克/千克体重的 AgNP 不会改变小鼠肠道微生物群的成员、结构或多样性。因此，与广谱抗生素的作用相反，AgNP 的重复给药剂量相当于口服参考剂量的 2000 倍和体外有效抗菌浓度的 100-400 倍，不会影响本地小鼠肠道微生物组。
Silver nanoparticles (AgNPs) have been used as antimicrobials in a number of applications, including topical wound dressings and coatings for consumer products & biomedical devices. Ingestion is a relevant route of exposure for AgNPs, whether occurring unintentionally via Ag dissolution from consumer products, or intentionally from dietary supplements. AgNP have also been proposed as substitutes for antibiotics in animal feeds. While oral antibiotics are known to have significant effects on gut bacteria, the antimicrobial effects of ingested AgNPs on the indigenous microbiome or on gut pathogens are unknown. Additionally, AgNP size and coating have been postulated as significantly influential towards their biochemical properties and the influence of these properties on antimicrobial efficacy is unknown. We evaluated murine gut microbial communities using culture-independent sequencing of 16S rRNA gene fragments following 28 days of repeated oral dosing of well-characterized AgNPs of two different sizes (20 and 110 nm) and coatings (PVP and Citrate). Irrespective of size or coating, oral administration of AgNPs at 10 mg/kg body weight/day did not alter the membership, structure, or diversity of the murine gut microbiome. Thus, in contrast to effects of broad-spectrum antibiotics, repeat dosing of AgNP, at doses equivalent to 2000 times the oral reference dose and 100–400 times the effective in vitro anti-microbial concentration, does not affect the indigenous murine gut microbiome.
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DOI：
10.1504/ijbnn.2013.054515
发表时间：
2013
期刊：
International journal of biomedical nanoscience and nanotechnology
影响因子：
--
作者：
Bergin IL;Witzmann FA
通讯作者：
Witzmann FA
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
3.9
作者：
Li, Xuan;Lenhart, John J.;Walker, Harold W.
通讯作者：
Walker, Harold W.
影响因子：
3.3
作者：
McConnell, Emma L.;Basit, Abdul W.;Murdan, Suclaxshina
通讯作者：
Murdan, Suclaxshina
影响因子：
1.2
作者：
Maneewattanapinyo, Pattwat;Banlunara, Wijit;Kaewamatawong, Theerayuth
通讯作者：
Kaewamatawong, Theerayuth