SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis.
SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis.
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SIZ1 介导的 SEUSS 相扑修饰调节拟南芥的光形态发生
DOI：
10.1016/j.xplc.2020.100080
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发表时间：
2020-09-14
影响因子：
10.5
通讯作者：
Lin R
中科院分区：
文献类型：
--
作者：
Zhang X;Huai J;Liu S;Jin JB;Lin R
小泛素样修饰物（SUMO）翻译后修饰（SUMO化）在调节多种生物学过程中起着重要作用，但其在植物光信号通路中的功能和调控机制尚不清楚。SEUSS（SEU）是一个转录辅助调节因子，它整合了光和温度信号通路，从而调控拟南芥的生长发育。在这里，我们表明，SEU是SUMO 1的底物，四个保守的赖氨酸残基的取代破坏SUMO化的SEU，损害其功能的光和热形态发生，并增强其与光敏色素相互作用因子4转录因子的相互作用。此外，SUMO E3连接酶SIZ1与SEU相互作用并调节其SUMO化。此外，SEU直接与光敏色素B光受体相互作用，并且SEU的SUMO化和稳定性被光激活。我们的研究揭示了一种新的SEU翻译后修饰机制，其中光通过SUMO化介导的蛋白质稳定性来调节植物的生长和发育。本研究表明，转录调节因子SEU与SIZ 1 SUMO E3连接酶和光敏色素B感光体相互作用，揭示了SEU的一种新的翻译后修饰机制，即光通过SUMO化介导的蛋白质稳定性调节植物生长发育。
Small ubiquitin-like modifier (SUMO) post-translational modification (SUMOylation) plays essential roles in regulating various biological processes; however, its function and regulation in the plant light signaling pathway are largely unknown. SEUSS (SEU) is a transcriptional co-regulator that integrates light and temperature signaling pathways, thereby regulating plant growth and development in Arabidopsis thaliana. Here, we show that SEU is a substrate of SUMO1, and that substitution of four conserved lysine residues disrupts the SUMOylation of SEU, impairs its function in photo- and thermomorphogenesis, and enhances its interaction with PHYTOCHROME-INTERACTING FACTOR 4 transcription factors. Furthermore, the SUMO E3 ligase SIZ1 interacts with SEU and regulates its SUMOylation. Moreover, SEU directly interacts with phytochrome B photoreceptors, and the SUMOylation and stability of SEU are activated by light. Our study reveals a novel post-translational modification mechanism of SEU in which light regulates plant growth and development through SUMOylation-mediated protein stability. This study shows that the transcriptional regulator SEU interacts with SIZ1 SUMO E3 ligase and phytochrome B photoreceptor and reveals a novel post-translational modification mechanism of SEU in which light regulates plant growth and development through SUMOylation-mediated protein stability.
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DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
DOI：
10.1073/pnas.0500778102
发表时间：
2005-05-24
影响因子：
11.1
作者：
Miura, K;Rus, A;Hasegawa, PM
通讯作者：
Hasegawa, PM
影响因子：
9.2
作者：
Leivar, Pablo;Monte, Elena;Oka, Yoshito;Liu, Tiffany;Carle, Christine;Castillon, Alicia;Huq, Enamul;Quail, Peter H.
通讯作者：
Quail, Peter H.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
拟南芥染色质重塑因子 PICKLE 与转录因子 HY5 相互作用调节下胚轴细胞伸长
影响因子：
11.6
作者：
Jing, Yanjun;Zhang, Dong;Lin, Rongcheng
通讯作者：
Lin, Rongcheng