Modulation of bioelectric cues in the evolution of flying fishes.
Modulation of bioelectric cues in the evolution of flying fishes.
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DOI：
10.1016/j.cub.2021.08.054
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发表时间：
2021-11-22
期刊：
影响因子：
9.2
通讯作者：
Harris, Matthew P.
中科院分区：
文献类型：
--
作者：
Daane, Jacob M.;Blum, Nicola;Lanni, Jennifer;Boldt, Helena;Iovine, M. Kathryn;Higdon, Charles W.;Johnson, Stephen L.;Lovejoy, Nathan R.;Harris, Matthew P.
异速生长的变化，或生物体内器官和组织的相对比例，是进化中适应性特征变化的一种常见手段。然而，关于相对大小是如何在发育过程中指定和在进化过程中形成的，人们知之甚少。在这里，通过对35种飞鱼及其近缘物种的全基因组变异的系统基因组分析，我们在编码区和调控区识别了导致成对鳍尺寸增加和空中滑翔行为收敛进化的遗传特征。为了完善我们的分析，我们将收敛的系统基因组特征与在远缘斑马鱼中发现的鳍大小改变的突变体进行了交叉。通过这些配对的方法，我们发现了L类型的氨基酸转运体lat4a和钾通道kcnh2a在FIN比例调节中的惊人作用。我们发现斑马鱼中这些遗传基因座之间的相互作用与观察到的飞鱼的鳍比例密切相关。实验和系统基因组研究结果的一致性表明，保守的、非正则的信号整合了生物电信号和氨基酸运输，在发育和进化中建立了相对大小。Daane等人。将飞鱼的基因组进化模式与斑马鱼的遗传筛选相交，以确定氨基酸转运体lat4a和钾通道kcnh2a在调节鳍大小方面的作用。这两个基因的等位基因组合足以在远亲斑马鱼身上复制飞鱼的鳍波普兰。
Changes to allometry, or the relative proportions of organs and tissues within organisms, is a common means for adaptive character change in evolution. However, little is understood about how relative size is specified during development and shaped during evolution. Here, through a phylogenomic analysis of genome-wide variation in 35 species of flying fishes and relatives, we identify genetic signatures in both coding and regulatory regions underlying the convergent evolution of increased paired fin size and aerial gliding behaviors. To refine our analysis, we intersected convergent phylogenomic signatures with mutants with altered fin size identified in distantly related zebrafish. Through these paired approaches, we identify a surprising role for an L-type amino acid transporter, lat4a, and the potassium channel, kcnh2a, in the regulation of fin proportion. We show that interaction between these genetic loci in zebrafish closely phenocopies the observed fin proportions of flying fishes. The congruence of experimental and phylogenomic findings point to conserved, non-canonical signaling integrating bioelectric cues and amino acid transport in the establishment of relative size in development and evolution. Daane et al. intersect patterns of genome evolution in flying fishes with genetic screens in zebrafish to identify a role for the amino acid transporter lat4a and the potassium channel kcnh2a in regulating fin size. A combination of alleles from both genes is sufficient to reproduce the flying fish fin bauplan in the distantly related zebrafish.
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