Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2.
Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2.
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普遍表达的转录物是激活信号转导蛋白抑制剂和转录激活蛋白 2 的蛋白抑制剂的新型相互作用蛋白。
DOI：
10.3892/mmr.2014.3023
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发表时间：
2015-04
影响因子：
3.4
通讯作者：
Zheng Y
中科院分区：
文献类型：
--
作者：
Kong X;Ma S;Guo J;Ma Y;Hu Y;Wang J;Zheng Y
活化信号换能器和转录激活因子2 （PIAS2）蛋白抑制剂是PIAS蛋白家族的成员。该蛋白家族调节几种转录因子的活性，并在sumo化途径中作为E3泛素连接酶。为了进一步了解PIAS2的生理作用，本研究使用酵母双杂交系统筛选小鼠干细胞cDNA文库中与PIAS2相互作用的蛋白。筛选发现PIAS2与泛在表达转录物（UXT）之间存在相互作用。UXT，也被称为雄激素受体捕获克隆-27，是α-类前折叠蛋白型伴侣，作为多种转录因子的共调节因子，包括核因子-κB和雄激素受体（AR）。通过酵母双杂交分析证实了PIAS2与UXT的直接互作。在体外，通过共免疫沉淀获得了UXT与PIAS2关联的证据。在HEK 293T和人宫颈癌HeLa细胞的细胞核和细胞质中发现了PIAS2和UXT的共定位。本研究结果表明，UXT是PIAS2的结合蛋白，PIAS2与UXT的相互作用可能对AR的转录激活起重要作用。
Protein inhibitor of activated signal transducer and activator of transcription 2 (PIAS2) is a member of the PIAS protein family. This protein family modulates the activity of several transcription factors and acts as an E3 ubiquitin ligase in the sumoylation pathway. To improve understanding of the physiological roles of PIAS2, the current study used a yeast two-hybrid system to screen mouse stem cell cDNA libraries for proteins that interact with PIAS2. The screening identified an interaction between PIAS2 and ubiquitously expressed transcript (UXT). UXT, also termed androgen receptor trapped clone-27, is an α-class prefoldin-type chaperone that acts as a coregulator for various transcription factors, including nuclear factor-κB and androgen receptor (AR). A direct interaction between PIAS2 and UXT was confirmed by direct yeast two-hybrid analysis. In vitro evidence of the association of UXT with PIAS2 was obtained by co-immunoprecipitation. Colocalization between PIAS2 and UXT was identified in the nucleus and cytoplasm of HEK 293T and human cervical carcinoma HeLa cells. The results of the current study suggested that UXT is a binding protein of PIAS2, and interaction between PIAS2 and UXT may be important for the transcriptional activation of AR.
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DOI：
10.1073/pnas.052559499
发表时间：
2002-03-05
影响因子：
11.1
作者：
Schmidt, D;Müller, S
通讯作者：
Müller, S
UXT-V1 通过调节复合物 II 的形成来保护细胞免受 TNF 诱导的细胞凋亡
影响因子：
3.3
作者：
Huang Y;Chen L;Zhou Y;Liu H;Yang J;Liu Z;Wang C
通讯作者：
Wang C
影响因子：
3.5
作者：
Santti, H;Mikkonen, L;Palvimo, JJ
通讯作者：
Palvimo, JJ
影响因子：
4.3
作者：
Zhang, Chao;Yuan, Xia;Yin, Zhimin
通讯作者：
Yin, Zhimin
DOI：
10.1007/s11626-007-9016-6
发表时间：
2007-04-01
影响因子：
2.1
作者：
Moss, Tijuana N.;Vo, Amy;Liu, Leyuan
通讯作者：
Liu, Leyuan