Origin of secretin receptor precedes the advent of tetrapoda: evidence on the separated origins of secretin and orexin.
Origin of secretin receptor precedes the advent of tetrapoda: evidence on the separated origins of secretin and orexin.
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DOI：
10.1371/journal.pone.0019384
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发表时间：
2011-04-29
期刊：
影响因子：
3.7
通讯作者：
Chow BK
中科院分区：
文献类型：
--
作者：
Tam JK;Lau KW;Lee LT;Chu JY;Ng KM;Fournier A;Vaudry H;Chow BK
目前，促胰液素及其受体仅在哺乳动物中被鉴定，这种配体-受体对在早期脊椎动物中的起源尚不清楚。此外，分泌素和食欲素在结构和功能方面的难以捉摸的相似性表明在脊椎动物谱系的早期有共同的祖先起源。本论文通过对肺鱼和黄鳍金枪鱼分泌素受体的克隆和功能研究，laevis和frog（X.和虎纹蛙）分泌素，我们提供的证据表明，分泌素配体受体对已经分歧，并成为高度具体的出现四足动物。从肺鱼中克隆的分泌素受体样序列表明，分泌素受体是在疥螨出现之前从VPAC样受体传下来的。为了阐明促胰液素和食欲素之间存在争议的关系，从X.光滑。我们证明，在青蛙中，分泌素和食欲素可以激活它们的相互受体，表明它们在非哺乳类脊椎动物中介导生理过程中的协调互补作用。然而，在促胰液素/胰高血糖素超家族中的肽中，促胰液素被发现是唯一可以激活食欲素受体的肽。因此，我们假设分泌素和食欲素是不同的祖先起源的早期脊椎动物谱系。
At present, secretin and its receptor have only been identified in mammals, and the origin of this ligand-receptor pair in early vertebrates is unclear. In addition, the elusive similarities of secretin and orexin in terms of both structures and functions suggest a common ancestral origin early in the vertebrate lineage. In this article, with the cloning and functional characterization of secretin receptors from lungfish and X. laevis as well as frog (X. laevis and Rana rugulosa) secretins, we provide evidence that the secretin ligand-receptor pair has already diverged and become highly specific by the emergence of tetrapods. The secretin receptor-like sequence cloned from lungfish indicates that the secretin receptor was descended from a VPAC-like receptor prior the advent of sarcopterygians. To clarify the controversial relationship of secretin and orexin, orexin type-2 receptor was cloned from X. laevis. We demonstrated that, in frog, secretin and orexin could activate their mutual receptors, indicating their coordinated complementary role in mediating physiological processes in non-mammalian vertebrates. However, among the peptides in the secretin/glucagon superfamily, secretin was found to be the only peptide that could activate the orexin receptor. We therefore hypothesize that secretin and orexin are of different ancestral origins early in the vertebrate lineage.
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影响因子：
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作者：
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DOI：
10.1073/pnas.0903695106
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