The serendipitous origin of chordate secretin peptide family members.
The serendipitous origin of chordate secretin peptide family members.
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DOI：
10.1186/1471-2148-10-135
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发表时间：
2010-05-06
影响因子：
3.4
通讯作者：
Power DM
中科院分区：
文献类型：
--
作者：
Cardoso JC;Vieira FA;Gomes AS;Power DM
促胰液素家族是脑肠肽的多效性组，其对2类G蛋白偶联受体（促胰液素家族GPCR）具有亲和力，提出其通过基因或基因组复制在后生动物辐射早期出现。在人类中，存在10个成员，并且序列和功能同源物以及配体-受体对已经在大多数脊椎动物类别的代表中被表征。分泌素样家族GPCR同源物也已在非脊椎动物基因组中分离，然而其相应的配体尚未令人信服地鉴定，并且其进化仍然是谜。计算机序列比较未能检索到非脊椎动物（多孔动物，刺胞动物，原口动物和早期后口动物）分泌素家族同源物。与此相反，分泌素家族成员被确定在七鳃鳗，几个硬骨鱼和四足动物和比较研究表明，序列和结构一般保持。序列比较和系统发育分析表明，PACAP、VIP和GCG是最高度保守的成员，存在两个主要的肽亚家族：i）PACAP-样，包括PACAP、PRP、VIP、PH、GHRH、SCT; ii）GCG-样，包括GCG、GLP 1、GLP 2和GIP。通过对东方鲀、非洲爪蟾、鸡和人类基因组的比较分析，建立了分泌素家族成员侧翼的保守区域，并在线虫、果蝇和玻璃海鞘基因组中鉴定了基因同源物，但没有发生基因连锁。然而，在果蝇和线虫中，位于脊椎动物分泌素家族成员侧翼的基因在同一染色体上被鉴定。促分泌素样家族GPCR的受体存在于原口动物中，但尚未鉴定出脊椎动物同源配体的序列同源物。目前还不可能确定配体是何时进化的，但似乎很可能是在原口-后口分化之后，通过基因/基因组重复，从现有基因或基因片段的一部分的外显子进化而来。重复外显子在不同的进化压力下起源于脊索动物PACAP样和GCG样亚家族。这一事件发生在后生动物分泌素GPCR的出现之后，并导致了新的肽-受体相互作用的建立，这些相互作用有助于在脊索动物谱系中产生新的生理功能。
The secretin family is a pleotropic group of brain-gut peptides with affinity for class 2 G-protein coupled receptors (secretin family GPCRs) proposed to have emerged early in the metazoan radiation via gene or genome duplications. In human, 10 members exist and sequence and functional homologues and ligand-receptor pairs have been characterised in representatives of most vertebrate classes. Secretin-like family GPCR homologues have also been isolated in non-vertebrate genomes however their corresponding ligands have not been convincingly identified and their evolution remains enigmatic. In silico sequence comparisons failed to retrieve a non-vertebrate (porifera, cnidaria, protostome and early deuterostome) secretin family homologue. In contrast, secretin family members were identified in lamprey, several teleosts and tetrapods and comparative studies revealed that sequence and structure is in general maintained. Sequence comparisons and phylogenetic analysis revealed that PACAP, VIP and GCG are the most highly conserved members and two major peptide subfamilies exist; i) PACAP-like which includes PACAP, PRP, VIP, PH, GHRH, SCT and ii) GCG-like which includes GCG, GLP1, GLP2 and GIP. Conserved regions flanking secretin family members were established by comparative analysis of the Takifugu, Xenopus, chicken and human genomes and gene homologues were identified in nematode, Drosophila and Ciona genomes but no gene linkage occurred. However, in Drosophila and nematode genes which flank vertebrate secretin family members were identified in the same chromosome. Receptors of the secretin-like family GPCRs are present in protostomes but no sequence homologues of the vertebrate cognate ligands have been identified. It has not been possible to determine when the ligands evolved but it seems likely that it was after the protostome-deuterostome divergence from an exon that was part of an existing gene or gene fragment by rounds of gene/genome duplication. The duplicate exon under different evolutionary pressures originated the chordate PACAP-like and GCG-like subfamily groups. This event occurred after the emergence of the metazoan secretin GPCRs and led to the establishment of novel peptide-receptor interactions that contributed to the generation of novel physiological functions in the chordate lineage.
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DOI：
10.1007/bf00315984
发表时间：
1991-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
ANDRIES, JC;BELEMTOUGRI, G;TRAMU, G
通讯作者：
TRAMU, G
影响因子：
3.4
作者：
Cardoso JC;de Vet EC;Louro B;Elgar G;Clark MS;Power DM
通讯作者：
Power DM
影响因子：
2.9
作者：
ANDRIES, JC;BELEMTOUGRI, G;TRAMU, G
通讯作者：
TRAMU, G
影响因子：
4.1
作者：
BERKS, BC;MARSHALL, CJ;CUTFIELD, JF
通讯作者：
CUTFIELD, JF
影响因子：
64.8
作者：
Delsuc, F;Brinkmann, H;Philippe, H
通讯作者：
Philippe, H