Optical magnetic imaging of living cells.
Optical magnetic imaging of living cells.
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作者：
磁成像是探测生物和物理系统的有力工具。然而，与光学显微镜相比，现有技术的空间分辨率较差，因此通常不适用于亚细胞结构的成像（例如磁共振成像[MRI]），或者需要的操作条件妨碍了在提供亚微米分辨率的同时应用于活体生物样品（例如扫描超导量子干涉装置[SQUID]显微镜，电子全息术和磁共振力显微镜[MRFM]）。在这里，我们展示了在实验室环境条件下，以亚细胞空间分辨率（400纳米）对活细胞（致磁细菌）的磁成像，使用由植入在金刚石芯片表面的纳米级氮空位（NV）色中心层组成的光学检测磁场成像阵列。将细菌放置在金刚石表面后，我们光学探测NV量子自旋态，并快速重建由细菌中产生的磁性纳米颗粒链（磁小体）产生的磁场矢量分量的图像，并将这些磁场图与在同一仪器中获得的光学图像在空间上联系起来。宽视场sCMOS采集允许在亚微米分辨率和bbb100微米视场下对种群中的多个细胞进行平行光学和磁成像。细菌的扫描电镜（SEM）图像证实了相关的光学和磁性图像可以用来定位和表征每种细菌的磁小体。研究结果为在高空间分辨率的环境条件下成像活细胞中的生物磁性结构提供了一种新的能力，并将使细胞和细胞网络内的大范围磁信号的映射成为可能。
Magnetic imaging is a powerful tool for probing biological and physical systems. However, existing techniques either have poor spatial resolution compared to optical microscopy and are hence not generally applicable to imaging of sub-cellular structure (e.g., magnetic resonance imaging [MRI]), or entail operating conditions that preclude application to living biological samples while providing sub-micron resolution (e.g., scanning superconducting quantum interference device [SQUID] microscopy, electron holography, and magnetic resonance force microscopy [MRFM]). Here we demonstrate magnetic imaging of living cells (magnetotactic bacteria) under ambient laboratory conditions and with sub-cellular spatial resolution (400 nm), using an optically-detected magnetic field imaging array consisting of a nanoscale layer of nitrogen-vacancy (NV) colour centres implanted at the surface of a diamond chip. With the bacteria placed on the diamond surface, we optically probe the NV quantum spin states and rapidly reconstruct images of the vector components of the magnetic field created by chains of magnetic nanoparticles (magnetosomes) produced in the bacteria, and spatially correlate these magnetic field maps with optical images acquired in the same apparatus. Wide-field sCMOS acquisition allows parallel optical and magnetic imaging of multiple cells in a population with sub-micron resolution and >100 micron field-of-view. Scanning electron microscope (SEM) images of the bacteria confirm that the correlated optical and magnetic images can be used to locate and characterize the magnetosomes in each bacterium. The results provide a new capability for imaging bio-magnetic structures in living cells under ambient conditions with high spatial resolution, and will enable the mapping of a wide range of magnetic signals within cells and cellular networks.
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影响因子：
3.6
作者：
Draper O;Byrne ME;Li Z;Keyhani S;Barrozo JC;Jensen G;Komeili A
通讯作者：
Komeili A
影响因子：
4
作者：
PROKSCH, RB;SCHAFFER, TE;FRANKEL, RB
通讯作者：
FRANKEL, RB
影响因子：
2.5
作者：
Lee, Seung-Cheol;Kim, Kiseong;Cheong, Chaejoon
通讯作者：
Cheong, Chaejoon
影响因子：
19.6
作者：
Grinolds, M. S.;Hong, S.;Yacoby, A.
通讯作者：
Yacoby, A.
DOI：
10.1073/pnas.1002562107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Hall, Liam T.;Hill, Charles D.;Hollenberg, Lloyd C. L.
通讯作者：
Hollenberg, Lloyd C. L.