Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance.
Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance.
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DOI：
10.1016/j.actbio.2011.11.025
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发表时间：
2012-03
影响因子：
9.7
通讯作者：
Okazaki, Masayuki
中科院分区：
文献类型：
--
作者：
Hirata, Isao;Yoshida, Yasuhiro;Nagaoka, Noriyuki;Hiasa, Kyou;Abe, Yasuhiko;Maekawa, Kenji;Kuboki, Takuo;Akagawa, Yasumasa;Suzuki, Kazuomi;Van Meerbeek, Bart;Messersmith, Phillip B.;Okazaki, Masayuki
钛的高耐腐蚀性和强度密度比使其广泛应用于主要工业，也广泛应用于医疗应用。在这里，我们报告的第一次，我们的钛钝化层传感器，利用表面等离子体共振（SPR）的发展。传感器上沉积的钛金属层在空气中钝化，就像钛医疗器械一样。我们的“Ti-SPR传感器”能够真实的实时分析生物分子与钛钝化表面的相互作用。作为概念验证，对暴露于酸的钛钝化层的腐蚀进行了真实的实时监测。此外，Ti-SPR传感器可以精确地测量蛋白质吸附到钛钝化层上的时间依赖性，精度为亚纳克/平方毫米。除了这种SPR分析，SPR成像（SPRI）能够实时评估在Ti-SPR传感器上的“多个独立点”同时发生的化学表面过程，例如酸腐蚀或细胞粘附。因此，我们的Ti-SPR传感器将是非常有用的研究钛腐蚀现象和生物分子钛表面的相互作用，在广泛的工业和生物医学领域的应用。
The high corrosion resistance and strength-to-density ratio makes titanium widely used in major industry, but also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, like titanium medical devices. Our ‘Ti-SPR sensor’ enables analysis of biomolecules interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of titanium passivation layer exposed to acid was monitored in real time. Also, the Ti-SPR sensor can accurately measure the time-dependence of protein adsorption onto titanium passivation layer with a sub-nanogram per square millimeter accuracy. Besides such SPR analyses, an SPR-imaging (SPRI) enables real-time assessment of chemical surface processes that occur simultaneously at ‘multiple independent spots’ on the Ti-SPR sensor, such as acid-corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium-corrosion phenomena and biomolecular titanium-surface interactions with application in a broad range of industrial and biomedical fields.
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影响因子：
4.4
作者：
Choi, WS;Kim, YM;Beaven, MA
通讯作者：
Beaven, MA
影响因子：
4.9
作者：
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Wennerberg, Ann
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Hirata, I;Hioko, Y;Iwata, H
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作者：
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通讯作者：
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