Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds.
Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds.
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DOI：
10.1002/term.442
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发表时间：
2012-05
影响因子：
3.3
通讯作者：
Hollinger, Jeffrey O.
中科院分区：
文献类型：
--
作者：
Kim, Jinku;Sharma, Aditi;Runge, Brett;Waters, Heather;Doll, Bruce;McBride, Sean;Alvarez, Pedro;Dadsetan, Mahrokh;Yaszemski, Michael J.;Hollinger, Jeffrey O.
关键词：
聚己内酯富马酸盐（PCLF）作为重组人骨形态发生蛋白2（rhBMP-2）的骨组织工程载体材料进行了研究。该制剂包括PCLF与聚（乙烯醇）（PVA）和羟基磷灰石（HA）的组合。评估包括体外和体内试验。体外试验使用前成骨细胞系（MC 3 T3-E1）验证了细胞附着。此外，从PCLF支架的rhBMP-2的体外释放曲线被确定长达21天。结果表明，PCLF复合PVA和HA可促进rhBMP-2的释放，释放的蛋白具有生物活性。对于体内研究，使用兔颅骨中的临界尺寸缺损（CSD）模型来测试PCLF支架。在植入后6周，在含有rhBMP-2的PCLF支架中测得的骨形成显著多于不含rhBMP-2的支架。总之，我们证明PCLF提供生物活性rhBMP-2，促进骨愈合在CSD中，并有潜力作为骨组织工程支架。
Poly(ε-caprolactone fumarate) (PCLF) scaffold formulations were assessed as a delivery system of recombinant human bone morphogenetic protein (rhBMP-2) for bone tissue engineering. The formulations included PCLF with combinations of poly(vinyl alcohol) (PVA) and hydroxyapatite (HA). The assessments included in vitro and in vivo assays. In vitro assays validated cell attachment using a pre-osteoblast cell line (MC3T3-E1). Additionally, in vitro release profiles of rhBMP-2 from PCLF scaffolds were determined up to 21 days. Data suggested PCLF incorporated with PVA and HA accelerated rhBMP-2 release and the released protein was bioactive. For the in vivo study, a critical sized defect (CSD) model in a rabbit calvaria was used to test PCLF scaffolds. At 6 weeks post-implantation, significantly more bone formation was measured in PCLF scaffolds containing rhBMP-2 than in scaffolds without rhBMP-2. In conclusion, we demonstrated PCLF delivered biologically active rhBMP-2, promoted bone healing in a CSD and has potential as a bone tissue engineering scaffold.
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影响因子：
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作者：
Magno, Maria Hanshella R.;Kim, Jinku;Kohn, Joachim
通讯作者：
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影响因子：
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