Elevations in vascular markers and eosinophils in chronic spontaneous urticarial weals with low-level persistence in uninvolved skin.
Elevations in vascular markers and eosinophils in chronic spontaneous urticarial weals with low-level persistence in uninvolved skin.
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DOI：
10.1111/bjd.12991
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Maurer M
中科院分区：
文献类型：
--
作者：
Kay AB;Ying S;Ardelean E;Mlynek A;Kita H;Clark P;Maurer M
在慢性自发性荨麻疹（CSU）中，肥大细胞活化与皮肤炎症变化一起被充分记录，并可能在组织水肿机制中发挥重要作用。通过测量病变和未受累皮肤中的微血管标记物、白细胞和肥大细胞数量来证实和扩展这些观察结果，并将结果与对照组进行比较。从8名CSU患者和9名对照受试者中采集了成对的活检组织（一份来自4-8小时的自发伤痕，一份来自未受累的皮肤），并使用凝集素荆豆凝集素1（UEA-1）进行免疫组织化学和共聚焦显微镜研究。CSU中的病变皮肤比非病变皮肤含有显著更多的CD 31+内皮细胞; CD 31+血管、中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和巨噬细胞;以及CD 3 + T细胞。通过使用凝集素UEA-1的共聚焦成像证实了血管分布增加。与对照组相比，CSU未受累皮肤含有更多的CD 31+内皮细胞、CD 31+血管和嗜酸性粒细胞。与对照组相比，CSU组肥大细胞数量增加了三倍，但在病变和未受累皮肤之间没有观察到差异。血管标记物增加以及嗜酸性粒细胞和中性粒细胞浸润是CSU病变皮肤的特征，可能导致组织水肿。嗜酸性粒细胞和微血管的变化在未受累的皮肤中持续存在，这与肥大细胞的增加一起表明，未受累的皮肤为进一步的磨损做好了准备。
In chronic spontaneous urticaria (CSU) mast cell activation together with inflammatory changes in the skin are well documented and may play an important role in mechanisms of tissue oedema. To confirm and extend these observations by measuring microvascular markers, leucocytes and mast cell numbers in lesional and uninvolved skin and to compare findings with a control group. Paired biopsies (one from 4–8-h spontaneous weals and one from uninvolved skin) were taken from eight patients with CSU and nine control subjects and studied using immunohistochemistry and confocal microscopy using the lectin Ulex europaeus agglutinin 1 (UEA-1). Lesional skin in CSU contained significantly more CD31+ endothelial cells; CD31+ blood vessels, neutrophils, eosinophils, basophils and macrophages; and CD3+ T cells than nonlesional skin. Increased vascularity was confirmed by confocal imaging using the lectin UEA-1. Uninvolved skin from CSU contained significantly more CD31+ endothelial cells, CD31+ blood vessels and eosinophils compared with the control subjects. There was a threefold increase in mast cell numbers when CSU was compared with controls but no difference was observed between lesional and uninvolved skin. Increased vascular markers together with eosinophil and neutrophil infiltration are features of lesional skin in CSU and might contribute to tissue oedema. Eosinophils and microvascular changes persist in uninvolved skin, which, together with increased mast cells, suggests that nonlesional skin is primed for further wealing.
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影响因子：
158.5
作者：
HIDE, M;FRANCIS, DM;GREAVES, MW
通讯作者：
GREAVES, MW
影响因子：
158.5
作者：
LEIFERMAN, KM;ACKERMAN, SJ;GLEICH, GJ
通讯作者：
GLEICH, GJ
影响因子：
2.9
作者：
Williams, MJ;Lowrie, MB;Clark, P
通讯作者：
Clark, P
影响因子：
64.8
作者：
WEDMORE, CV;WILLIAMS, TJ
通讯作者：
WILLIAMS, TJ
影响因子：
9.6
作者：
Hashimoto, M;Tanaka, H;Abe, S
通讯作者：
Abe, S