Activation of an innate immune response in the schistosome-transmitting snail Biomphalaria glabrata by specific bacterial PAMPs.
Activation of an innate immune response in the schistosome-transmitting snail Biomphalaria glabrata by specific bacterial PAMPs.
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DOI：
10.1016/j.dci.2013.09.016
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发表时间：
2014-02
影响因子：
2.9
通讯作者：
Belloir, Joseph A.
中科院分区：
文献类型：
--
作者：
Sullivan, John T.;Belloir, Joseph A.
将大肠杆菌粗脂多糖（LPS）注入光滑双脐螺（Biomphalaria glabrata）血腔，刺激产阿米巴器官（APO）细胞增殖。然而，尚不清楚促有丝分裂活性是否存在于LPS的脂质A或O-多糖组分中。此外，通常污染粗LPS和已知刺激哺乳动物先天免疫应答的物质的可能作用，肽聚糖（PGN），蛋白质或细菌DNA，尚不清楚。因此，我们测试了以下注射物质对蜗牛APO的影响：粗LPS、超纯化LPS（无脂蛋白污染）、两种形式的脂质A（二磷酰脂质A和Kdo 2-脂质A）、O-多糖、革兰氏阴性PGN（粗品和超纯化的）（分别具有和不具有内毒素活性）、革兰氏阳性PGN、PGN组分Tri-DAP和胞壁酰二肽以及细菌DNA。而粗LPS，超纯化的LPS，和粗PGN是促有丝分裂，超纯化的PGN不是。此外，LPS组分、PGN组分和细菌DNA无活性。这些结果表明，它是完整的LPS分子，刺激细胞分裂的载脂蛋白O。
Injection of crude lipopolysaccharide (LPS) from Eschericia coli into the hemocoel of Biomphalaria glabrata stimulates cell proliferation in the amebocyte-producing organ (APO). However, it is not known if mitogenic activity resides in the lipid A or O-polysaccharide component of LPS. Moreover, the possible role of substances that commonly contaminate crude LPS and that are known to stimulate innate immune responses in mammals, e.g., peptidoglycan (PGN), protein, or bacterial DNA, is unclear. Therefore, we tested the effects of the following injected substances on the snail APO: crude LPS, ultrapurified LPS (lacking lipoprotein contamination), two forms of lipid A, (diphosphoryl lipid A and Kdo2-lipid A), O-polysaccharide, Gram negative PGN, both crude and ultrapurified (with and without endotoxin activity, respectively), Gram positive PGN, PGN components Tri-DAP and muramyl dipeptide, and bacterial DNA. Whereas crude LPS, ultrapurified LPS, and crude PGN were mitogenic, ultrapurified PGN was not. Moreover, LPS components, PGN components, and bacterial DNA were inactive. These results suggest that it is the intact LPS molecule which stimulates cell division in the APO.
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影响因子：
4.7
作者：
Hong, Xu-Tao;Xiang, Li-Xin;Shao, Jian-Zhong
通讯作者：
Shao, Jian-Zhong
影响因子：
2.9
作者：
Sullivan, John T.;Bulman, Christina A.;Salamat, Zahra
通讯作者：
Salamat, Zahra
影响因子：
3.6
作者：
MacKenzie, Simon A.;Roher, Nerea;Goetz, Frederick W.
通讯作者：
Goetz, Frederick W.
影响因子：
32.4
作者：
Kaneko, T;Goldman, WE;Silverman, N
通讯作者：
Silverman, N
影响因子：
4.4
作者：
Gao, JJ;Xue, Q;Morrison, DC
通讯作者：
Morrison, DC