The evolution of the phage shock protein response system: interplay between protein function, genomic organization, and system function.
The evolution of the phage shock protein response system: interplay between protein function, genomic organization, and system function.
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DOI：
10.1093/molbev/msq301
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发表时间：
2011-03
影响因子：
10.7
通讯作者：
Stumpf MP
中科院分区：
文献类型：
--
作者：
Huvet M;Toni T;Sheng X;Thorne T;Jovanovic G;Engl C;Buck M;Pinney JW;Stumpf MP
感知环境并对其做出适当的反应是生物体生存的关键能力。所有现存的生物都必须进化出合适的传感器、信号系统和反应机制，使它们能够在可能遇到的条件下生存。在这里，我们详细研究了这样一个系统的进化史：噬菌体休克蛋白（Psp）应激反应系统是许多细菌（包括大肠杆菌K12）应激反应机制的重要组成部分。在这里，我们对大肠杆菌Psp系统的组成和调控基因进行了系统分析，以阐明该系统的进化史。我们比较了蛋白质编码和非编码DNA序列的基因共享、序列进化和保守性，并将这些与698种细菌基因组的基因组/操纵子组织的比较分析联系起来。最后，我们通过实验评估了简化版Psp系统在不同氧相关环境下的生物学优势/劣势。我们的研究结果表明，Psp系统是围绕一个核心反应机制进化的，通过逐渐将基因纳入系统，提供更细微的感觉、信号和效应功能。我们发现，新基因进入应答机制与这些基因进入psp操纵子密切相关，正如在大肠杆菌中看到的那样，其中包含了参与应答的大部分基因。这个操纵子的组织允许令人惊讶的水平额外的转录控制和灵活性。这里讨论的结果表明，只有在系统的整体功能得以维持的情况下，这些信号系统的组成部分才会在进化上保持保守。
Sensing the environment and responding appropriately to it are key capabilities for the survival of an organism. All extant organisms must have evolved suitable sensors, signaling systems, and response mechanisms allowing them to survive under the conditions they are likely to encounter. Here, we investigate in detail the evolutionary history of one such system: The phage shock protein (Psp) stress response system is an important part of the stress response machinery in many bacteria, including Escherichia coli K12. Here, we use a systematic analysis of the genes that make up and regulate the Psp system in E. coli in order to elucidate the evolutionary history of the system. We compare gene sharing, sequence evolution, and conservation of protein-coding as well as noncoding DNA sequences and link these to comparative analyses of genome/operon organization across 698 bacterial genomes. Finally, we evaluate experimentally the biological advantage/disadvantage of a simplified version of the Psp system under different oxygen-related environments. Our results suggest that the Psp system evolved around a core response mechanism by gradually co-opting genes into the system to provide more nuanced sensory, signaling, and effector functionalities. We find that recruitment of new genes into the response machinery is closely linked to incorporation of these genes into a psp operon as is seen in E. coli, which contains the bulk of genes involved in the response. The organization of this operon allows for surprising levels of additional transcriptional control and flexibility. The results discussed here suggest that the components of such signaling systems will only be evolutionarily conserved if the overall functionality of the system can be maintained.
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影响因子：
5.6
作者：
Joly N;Burrows PC;Engl C;Jovanovic G;Buck M
通讯作者：
Buck M
影响因子：
3.2
作者：
Jovanovic, G;Dworkin, J;Model, P
通讯作者：
Model, P
DOI：
10.1038/nrmicro1949
发表时间：
2009-02
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1126/stke.3442006re6
发表时间：
2006-07-18
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
--
作者：
Hlavacek, William S;Faeder, James R;Fontana, Walter
通讯作者：
Fontana, Walter
影响因子：
2.6
作者：
Fondi, Marco;Emiliani, Giovanni;Fani, Renato
通讯作者：
Fani, Renato