Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field.
Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field.
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DOI：
10.1093/nar/gkl035
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发表时间：
2006
影响因子：
14.9
通讯作者：
Hottiger MO
中科院分区：
文献类型：
--
作者：
Kamau SW;Hassa PO;Steitz B;Petri-Fink A;Hofmann H;Hofmann-Amtenbrink M;von Rechenberg B;Hottiger MO
仍然需要新的方法来提高非病毒基因传递的效率。在这里，我们报告了一种简单的方法，增强基因传递使用永久和脉动磁场。将含有绿色荧光蛋白编码序列的DNA质粒和新型DNA片段（PCR产物）偶联到聚乙烯亚胺包被的超顺磁性纳米颗粒（SPIONs）上。这些复合物被添加到细胞中，随后暴露在永久和脉动磁场中。这些磁场的存在使转染效率比未暴露于磁场的细胞显著提高了40倍。无论是小颗粒（50 nm）还是大颗粒（200 ~ 250 nm），在脉动场作用前将纳米颗粒沉积在永磁体上时，转染效率最高。5分钟内高效的基因转移表明，该技术是未来体内研究的有力工具，在基因载体发生系统清除或过滤之前，需要快速的基因传递。
New approaches to increase the efficiency of non-viral gene delivery are still required. Here we report a simple approach that enhances gene delivery using permanent and pulsating magnetic fields. DNA plasmids and novel DNA fragments (PCR products) containing sequence encoding for green fluorescent protein were coupled to polyethylenimine coated superparamagnetic nanoparticles (SPIONs). The complexes were added to cells that were subsequently exposed to permanent and pulsating magnetic fields. Presence of these magnetic fields significantly increased the transfection efficiency 40 times more than in cells not exposed to the magnetic field. The transfection efficiency was highest when the nanoparticles were sedimented on the permanent magnet before the application of the pulsating field, both for small (50 nm) and large (200–250 nm) nanoparticles. The highly efficient gene transfer already within 5 min shows that this technique is a powerful tool for future in vivo studies, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.
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影响因子：
5.1
作者：
Hildebrandt, IJ;Iyer, M;Gambhir, SS
通讯作者：
Gambhir, SS
影响因子：
5.1
作者：
Ogris, M;Steinlein, P;Wagner, E
通讯作者：
Wagner, E
影响因子：
2.7
作者：
Schöpf, B;Neuberger, T;von Rechenberg, B
通讯作者：
von Rechenberg, B
影响因子：
5.1
作者：
Aigner, A;Fischer, D;Czubayko, F
通讯作者：
Czubayko, F
DOI：
10.1016/j.ejpb.2004.11.011
发表时间：
2005-07-01
影响因子：
4.9
作者：
Lungwitz, U;Breunig, M;Göpferich, A
通讯作者：
Göpferich, A