Dynamic reorganization of open chromatin underlies diverse transcriptomes during spermatogenesis.
Dynamic reorganization of open chromatin underlies diverse transcriptomes during spermatogenesis.
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开放染色质的动态重组是精子发生过程中各种转录组的基础。
DOI：
10.1093/nar/gkx1052
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发表时间：
2018-01-25
影响因子：
14.9
通讯作者：
Namekawa SH
中科院分区：
文献类型：
--
作者：
Maezawa S;Yukawa M;Alavattam KG;Barski A;Namekawa SH
During spermatogenesis, germ cells undergo massive cellular reconstruction and dynamic chromatin remodeling to facilitate highly diverse transcriptomes, which are required for the production of functional sperm. However, it remains unknown how germline chromatin is organized to promote the dynamic, complex transcriptomes of spermatogenesis. Here, using ATAC-seq, we establish the varied landscape of open chromatin during spermatogenesis. We identify the reorganization of accessible chromatin in intergenic and intronic regions during the mitosis-to-meiosis transition. During the transition, mitotic-type open chromatin is closed while the de novo formation of meiotic-type open chromatin takes place. Contrastingly, differentiation processes such as spermatogonial differentiation and the meiosis-to-postmeiosis transition involve chromatin closure without the de novo formation of accessible chromatin. In spermiogenesis, the germline-specific Polycomb protein SCML2 promotes the closure of open chromatin at autosomes for gene suppression. Paradoxically, we identify the massive de novo formation of accessible chromatin when the sex chromosomes undergo meiotic sex chromosome inactivation, and this is also mediated by SCML2. These results reveal meiotic sex chromosome inactivation as an active process for chromatin organization. Together, our results unravel the genome-wide, dynamic reorganization of open chromatin and reveal mechanisms that underlie diverse transcriptomes during spermatogenesis.
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影响因子：
4.5
作者：
Gely-Pernot A;Raverdeau M;Teletin M;Vernet N;Féret B;Klopfenstein M;Dennefeld C;Davidson I;Benoit G;Mark M;Ghyselinck NB
通讯作者：
Ghyselinck NB
影响因子：
16.8
作者：
Brykczynska, Urszula;Hisano, Mizue;Peters, Antoine H. F. M.
通讯作者：
Peters, Antoine H. F. M.
影响因子：
30.8
作者：
Khil, PP;Smirnova, NA;Camerini-Otero, RD
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Camerini-Otero, RD
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12.3
作者：
Kartashov AV;Barski A
通讯作者：
Barski A
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS