Evaluation of inflammation and follicle depletion during ovarian ageing in mice.
Evaluation of inflammation and follicle depletion during ovarian ageing in mice.
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DOI：
10.1038/s41598-020-79488-4
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发表时间：
2021-01-11
影响因子：
4.6
通讯作者：
Hutt K
中科院分区：
文献类型：
--
作者：
Lliberos C;Liew SH;Zareie P;La Gruta NL;Mansell A;Hutt K
女性生殖老化的定义是卵泡数量和卵母细胞质量的逐渐下降。这是一个自然过程，导致生育能力和卵巢功能丧失，周期不规则，最终绝经或生殖衰老。在整个生殖生命中卵泡自然损耗的因素是不明确的。有人提出，炎症过程和纤维化可能有助于卵巢老化。为了进一步研究这种可能性，我们评估了2、6、12和18月龄C57BL/6雌性小鼠卵巢中炎症和免疫细胞群的关键标志物。我们报道，卵泡数量的减少与卵巢内CD4 + T细胞、B细胞和巨噬细胞百分比的增加有关。血清中IL-1α/β、TNF-α、IL-6、炎性小体基因ASC和NLRP3等促炎因子的浓度和卵巢内mRNA水平均随年龄的增长而显著升高。通过胶原I和III的小天狼星红染色测定的纤维化水平直到18个月大时都没有变化。总的来说，这些数据表明炎症可能是与年龄相关的卵泡数量调节机制之一，但纤维化的作用尚不清楚。现在需要进一步的研究来确定随着女性年龄的增长，炎症和卵泡衰竭之间是否存在因果关系。
Reproductive ageing in females is defined by a progressive decline in follicle number and oocyte quality. This is a natural process that leads to the loss of fertility and ovarian function, cycle irregularity and eventually menopause or reproductive senescence. The factors that underlie the natural depletion of follicles throughout reproductive life are poorly characterised. It has been proposed that inflammatory processes and fibrosis might contribute to ovarian ageing. To further investigate this possibility, we evaluated key markers of inflammation and immune cell populations in the ovaries of 2, 6, 12 and 18-month-old C57BL/6 female mice. We report that the decrease in follicle numbers over the reproductive lifespan was associated with an increase in the intra-ovarian percentage of CD4 + T cells, B cells and macrophages. Serum concentration and intra-ovarian mRNA levels of several pro-inflammatory cytokines, including IL-1α/β, TNF-α, IL-6, and inflammasome genes ASC and NLRP3, were significantly increased with age. Fibrosis levels, as determined by picrosirius red staining for collagen I and III, were unchanged up to 18 months of age. Collectively, these data suggest that inflammation could be one of the mechanisms responsible for the age-related regulation of follicle number, but the role of fibrosis is unclear. Further studies are now required to determine if there is a causative relationship between inflammation and follicle depletion as females age.
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DOI：
10.1530/rep-07-0229
发表时间：
2007-11
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Herath S;Williams EJ;Lilly ST;Gilbert RO;Dobson H;Bryant CE;Sheldon IM
通讯作者：
Sheldon IM
DOI：
10.1084/jem.20082039
发表时间：
2008-12-22
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Iijima N;Linehan MM;Zamora M;Butkus D;Dunn R;Kehry MR;Laufer TM;Iwasaki A
通讯作者：
Iwasaki A
影响因子：
7.3
作者：
Comi M;Amodio G;Gregori S
通讯作者：
Gregori S
影响因子：
4.6
作者：
Lliberos C;Liew SH;Zareie P;La Gruta NL;Mansell A;Hutt K
通讯作者：
Hutt K
DOI：
10.1530/rep-16-0129
发表时间：
2016-09
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Briley SM;Jasti S;McCracken JM;Hornick JE;Fegley B;Pritchard MT;Duncan FE
通讯作者：
Duncan FE