Detection of high frequency of MAD20 allelic variants of Plasmodium falciparum merozoite surface protein 1 gene from Adama and its surroundings, Oromia, Ethiopia.
Detection of high frequency of MAD20 allelic variants of Plasmodium falciparum merozoite surface protein 1 gene from Adama and its surroundings, Oromia, Ethiopia.
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DOI：
10.1186/s12936-021-03914-9
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发表时间：
2021-09-27
期刊：
影响因子：
3
通讯作者：
Golassa L
中科院分区：
文献类型：
--
作者：
File T;Chekol T;Solomon G;Dinka H;Golassa L
开发针对恶性疟原虫无性阶段的有效疫苗的主要挑战之一是寄生虫种群内的遗传多态性。了解恶性疟原虫裂殖子表面蛋白 1 (msp-1) 基因的区块 2 区域等遗传多态性，有助于阐明疾病病理学的机制、从分离株中鉴定寄生虫克隆特征、传播强度以及当地正在进行的疟疾控制和消除工作的潜在缺陷。对当地遗传多态性的详细了解可以为更好地管理、控制和消除疟疾铺平道路。本研究的目的是检测来自埃塞俄比亚奥罗米亚州阿达玛镇及其周边地区选定卫生机构的恶性疟原虫临床分离株的 msp-1 基因最常见的等位基因变异。使用特异性引物成功扩增了 139 个临床分离株的 msp-1 基因。针对 K1、MAD20 和 R033 等位基因进行巢式 PCR 扩增，然后进行凝胶电泳进行片段分析。根据 PCR 片段的检测，感染被分为单克隆感染或多重感染。本研究共鉴定出19个不同大小的msp-1基因多态性，其中MAD20等位基因家族67个（48%），K-1等位基因家族18个（13%），RO33等位基因家族18个（13%）。而MAD20 + K-1、MAD20 + RO33、K-1 + RO33和MAD20 + K-1、RO33的多重感染分别为21例（15%）、8例（5.8%）、4例（2.9%）和3例（2.2%）。总体感染复数 (MOI) 为 1.3，预期杂合度 (He) 为 0.39，表明恶性疟疾传播略低。研究中观察到的msp-1等位基因大小多态性、MOI和He的状态表明恶性疟原虫临床分离株存在略低的遗传多样性。然而，在研究区域的临床分离株中检测到了高频率的 MAD20 等位基因变异。此外，在疟疾衰退地区揭示的导致 MAD20 等位基因变异高度优势的驱动力需要进一步研究。在线版本包含可在 10.1186/s12936-021-03914-9 获取的补充材料。
One of the major challenges in developing an effective vaccine against asexual stages of Plasmodium falciparum is genetic polymorphism within parasite population. Understanding the genetic polymorphism like block 2 region of merozoite surface protein-1 (msp-1) gene of P. falciparum enlighten mechanisms underlining disease pathology, identification of the parasite clone profile from the isolates, transmission intensity and potential deficiencies of the ongoing malaria control and elimination efforts in the locality. Detailed understanding of local genetic polymorphism is an input to pave the way for better management, control and elimination of malaria. The aim of this study was to detect the most frequent allelic variant of the msp-1 gene of P. falciparum clinical isolates from selected health facilities in Adama town and its surroundings, Oromia, Ethiopia. One hundred thirty-nine clinical isolates were successfully amplified for msp-1 gene using specific primers. Nested PCR amplification was conducted targeting K1, MAD20, and R033 alleles followed by gel electrophoresis for fragment analysis. Based on the detection of a PCR fragment, infections were classified as monoclonal or multiple infections. 19 different size polymorphism of msp-1 gene were identified in the study, with 67(48%) MAD20, 18 (13%) K-1 and 18 (13%) RO33 allelic family. Whereas, the multiple infections were 21(15%), 8 (5.8%), 4(2.9%), 3(2.2%) for MAD20 + K-1, MAD20 + RO33, K-1 + RO33, and MAD20 + K-1, RO33, respectively. The overall Multiplicity of infection (MOI) was 1.3 and the expected heterozygosity (He) was 0.39 indicating slightly low falciparum malaria transmission. The status of msp-1 allele size polymorphism, MOI and He observed in the study revealed the presence of slightly low genetic diversity of P. falciparum clinical isolates. However, highly frequent MAD20 allelic variant was detected from clinical isolates in the study area. Moreover, the driving force that led to high predominance of MAD20 allelic variant revealed in such malaria declining region demands further research. The online version contains supplementary material available at 10.1186/s12936-021-03914-9.
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