Genetic diversity of Plasmodium falciparum isolates based on MSP-1 and MSP-2 genes from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia.
Genetic diversity of Plasmodium falciparum isolates based on MSP-1 and MSP-2 genes from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia.
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DOI：
10.1186/s12936-015-0604-8
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发表时间：
2015-02-14
期刊：
影响因子：
3
通讯作者：
Kebede A
中科院分区：
文献类型：
--
作者：
Mohammed H;Mindaye T;Belayneh M;Kassa M;Assefa A;Tadesse M;Woyessa A;Mengesha T;Kebede A
恶性疟原虫的遗传多样性已在各国得到广泛研究。然而，埃塞俄比亚提供的数据有限。本研究旨在评估埃塞俄比亚西南部Kolla-Shele地区恶性疟原虫的遗传多样性程度。2008年9月至12月，从Kolla-Shele健康中心的无并发症恶性疟原虫患者中共收集了88株分离株。在通过Chelex®方法提取DNA后，通过使用mspl（区块2）和msp 2（区块3）的巢式PCR对样品进行基因分型，包括它们的等位基因家族：K1、MAD 20、R 033和FC 27、3D 7/IC 1。在88份血液样本中发现了msp 1和msp 2的等位基因变异。观察到msp 1 67%（59/88）和msp 2 44%（39/88）。K1是在33.9%（20/59）的样本中观察到的主要msp 1等位基因家族，其次是RO 33和MAD 20。在msp 2等位基因家族中，3D 7/IC 1的频率（21.5%）高于FC 27（10.3%）。共检测到23个等位基因，其中11个来自msp 2基因，12个来自msp 2基因。59%的分离株具有多种基因型，总体平均感染复数为1.8（95%CI：1.48-2.04）。msp 1和msp 2的杂合度分别为0.79和0.54。感染复数与年龄、虫体密度的差异均无统计学意义（P > 0.05）。该遗传多样性研究表明，在研究区域存在5个等位基因类型，在msp 1家族和3D 7/IC 1在msp 2家族中的优势K1。在近60%的样本中观察到多重感染。本文的在线版本（doi：10.1186/s12936-015-0604-8）包含补充材料，可供授权用户使用。
The genetic diversity of Plasmodium falciparum has been extensively studied in various countries. However, limited data are available from Ethiopia. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Kolla-Shele, in the southwest of Ethiopia. A total of 88 isolates from patients with uncomplicated P. falciparum attending Kolla-Shele Health Centre was collected from September to December, 2008. After extraction of DNA by Chelex® method, the samples were genotyped by using nested-PCR of msp1 (block 2) and msp2 (block 3) including their allelic families: K1, MAD20, RO33 and FC27, 3D7/IC1, respectively. Allelic variation in both msp1 and msp2 were identified in the 88 blood samples. For msp1 67% (59/88) and msp2 44% (39/88) were observed. K1 was the predominant msp1 allelic family observed in 33.9% (20/59) of the samples followed by RO33 and MAD20. Of the msp2 allelic family 3D7/IC1 showed higher frequency (21.5%) compared to FC27 (10.3%). A total of twenty-three alleles were detected; of which, eleven were from msp2 and twelve from msp2 genes. Fifty-nine percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.8 (95% CI: 1.48-2.04). The heterozygosity index was 0.79 and 0.54for msp1 and msp2, respectively. There was no statically significant difference in the multiplicity of infection by either age or parasite density (P > 0.05). This genetic diversity study showed the presence of five allelic types in the study area, with dominance K1 in the msp1 family and 3D7/IC1 in the msp2 family. Multiple infections were observed in nearly 60% of the samples. The online version of this article (doi:10.1186/s12936-015-0604-8) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Soulama I;Nébié I;Ouédraogo A;Gansane A;Diarra A;Tiono AB;Bougouma EC;Konaté AT;Kabré GB;Taylor WR;Sirima SB
通讯作者：
Sirima SB
影响因子：
2.8
作者：
HOLDER, AA;BLACKMAN, MJ;SHAI, S
通讯作者：
SHAI, S
影响因子：
3.3
作者：
Peyerl-Hoffmann, G;Jelinek, T;von Sonnenburg, F
通讯作者：
von Sonnenburg, F
影响因子：
2.1
作者：
Ferreira, Marcelo U.;Hartl, Daniel L.
通讯作者：
Hartl, Daniel L.
DOI：
10.4269/ajtmh.12-0745
发表时间：
2013-09
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Massebo F;Balkew M;Gebre-Michael T;Lindtjørn B
通讯作者：
Lindtjørn B