Arabidopsis MYB24 Regulates Jasmonate-Mediated Stamen Development.

Arabidopsis MYB24 Regulates Jasmonate-Mediated Stamen Development.
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拟南芥 MYB24 调节茉莉酸介导的雄蕊发育

DOI:
10.3389/fpls.2017.01525
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发表时间:
2017
影响因子:
5.6
通讯作者:
Song S
Song S
中科院分区:
生物学2区
文献类型:
--
作者:
Huang H;Gao H;Liu B;Qi T;Tong J;Xiao L;Xie D;Song S

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植物激素茉莉酸(Jas)调节各种防御反应和不同的发育过程,包括雄蕊发育和育性。以往的研究表明,茉莉酸诱导JA ZIM结构域(JAZ)蛋白的降解,并激活MYB转录因子(如MYB21和MYB24)来调节雄蕊的发育。在本研究中,我们进一步揭示了MYB24与JAZ相互作用控制JA调控雄蕊发育的机制。我们发现MYB21/24的N-端与12个JaZ蛋白中的10个相互作用,而MYB24的N-端和C-端都参与了MYB21和MYB24的二聚化。有趣的是,JA缺失突变体opr3的雄性不育可以通过适当水平的MYB24过表达来挽救,但不能通过过高水平的MYB24来挽救。令人惊讶的是,MYB24NT的过度表达而不是MYB24CT的过度表达可能导致男性不育。这些结果为研究茉莉酸调控雄蕊发育中的MYB因子提供了新的见解。
The phytohormone jasmonates (JAs) regulate various defense responses and diverse developmental processes including stamen development and fertility. Previous studies showed that JA induces CORONATINE INSENSITIVE 1-mediated degradation of JA ZIM-domain (JAZ) proteins, and activates the MYB transcription factors (such as MYB21 and MYB24) to regulate stamen development. In this study, we further uncover the mechanism underlying how MYB24 interacts with JAZs to control JA-regulated stamen development. We show that N-terminus of MYB21/24 interacts with 10 out of 12 JAZ proteins while both N-terminus and C-terminus of MYB24 are involved in dimerization of MYB21 and MYB24. Interestingly, male sterility of the JA-deficient mutant opr3 can be rescued by suitable level of the MYB24 overexpression but not by excessive high level of MYB24. Surprisingly, overexpression of MYB24NT, but not MYB24CT, could cause male sterility. These results provide new insights on MYB factors in JA-regulated stamen development.
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