Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis.
Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis.
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DOI：
10.1093/jxb/erq408
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Browse J
中科院分区：
文献类型：
--
作者：
Niu Y;Figueroa P;Browse J
植物激素茉莉酸（JA）在植物防御和生长发育中起着重要的调节作用。JAZ蛋白抑制调节早期JA-responsive基因的转录因子，因此JAZ蛋白的JA-induced降解允许这些应答基因的表达。迄今为止，MYC2是已知的唯一直接与JAZ蛋白相互作用并调节早期JA反应的转录因子，但myc2突变体的表型表明其他转录因子也激活JA反应。为了鉴定JAZ1相互作用蛋白，进行拟南芥cDNA文库的酵母双杂交筛选。鉴定了两种碱性螺旋-环-螺旋（bHLH）蛋白，MYC 3和MYC 4。MYC 3和MYC 4与MYC 2具有高度的序列相似性，提示它们可能具有相似的生物学功能。MYC3和MYC4不仅与JAZ1相互作用，而且在酵母双杂交和下拉测定中与其他JAZ蛋白（JAZ3和JAZ9）相互作用。MYC 2、MYC 3和MYC 4都能够在胡萝卜原生质体转染后诱导JAZ：：GUS报告构建体的表达。虽然myc3和myc4功能丧失突变体没有表现出表型，但与野生型植物相比，过表达MYC3和MYC4的转基因植物具有更高水平的花青素苷。此外，MYC3过表达植株的根对JA高度敏感。对9个JA响应基因的实时定量RT-PCR表达分析显示，其中8个在MYC3和MYC4过表达植物中被诱导，除了病原体响应基因PDF1. 2。与MYC2类似，MYC4负调控PDF1.2的表达。总之，这些结果表明，MYC3和MYC4是JAZ相互作用的转录因子，调节JA反应。
The plant hormone jasmonate (JA) plays important roles in the regulation of plant defence and development. JASMONATE ZIM-DOMAIN (JAZ) proteins inhibit transcription factors that regulate early JA-responsive genes, and JA-induced degradation of JAZ proteins thus allows expression of these response genes. To date, MYC2 is the only transcription factor known to interact directly with JAZ proteins and regulate early JA responses, but the phenotype of myc2 mutants suggests that other transcription factors also activate JA responses. To identify JAZ1-interacting proteins, a yeast two-hybrid screen of an Arabidopsis cDNA library was performed. Two basic helix-loop-helix (bHLH) proteins, MYC3 and MYC4, were identified. MYC3 and MYC4 share high sequence similarity with MYC2, suggesting they may have similar biological functions. MYC3 and MYC4 interact not only with JAZ1 but also with other JAZ proteins (JAZ3 and JAZ9) in both yeast two-hybrid and pull-down assays. MYC2, MYC3, and MYC4 were all capable of inducing expression of JAZ::GUS reporter constructs following transfection of carrot protoplasts. Although myc3 and myc4 loss-of-function mutants showed no phenotype, transgenic plants overexpressing MYC3 and MYC4 had higher levels of anthocyanin compared to the wild-type plants. In addition, roots of MYC3 overexpression plants were hypersensitive to JA. Quantitative real-time RT-PCR expression analysis of nine JA-responsive genes revealed that eight of them were induced in MYC3 and MYC4 overexpression plants, except for a pathogen-responsive gene, PDF1.2. Similar to MYC2, MYC4 negatively regulates expression of PDF1.2. Together, these results suggest that MYC3 and MYC4 are JAZ-interacting transcription factors that regulate JA responses.
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