The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs.
The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs.
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DOI：
10.1261/rna.044701.114
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Pillai RS
中科院分区：
文献类型：
--
作者：
Cora E;Pandey RR;Xiol J;Taylor J;Sachidanandam R;McCarthy AA;Pillai RS
This paper presents the crystal structure of the MID domain of a Piwi Argonaute protein. Docking experiments show that this domain specifies recognition of the 5′ uridine of piRNAs. Domain-swapping experiments reveal an unexpected role for the MID domain indicating strand orientation of its bound piRNA. Piwi-interacting RNAs (piRNAs) guide Piwi Argonautes to suppress transposon activity in animal gonads. Known piRNA populations are extremely complex, with millions of individual sequences present in a single organism. Despite this complexity, specific Piwi proteins incorporate piRNAs with distinct nucleotide- and transposon strand-biases (antisense or sense) of unknown origin. Here, we examined the contribution of structural domains in Piwi proteins toward defining these biases. We report the first crystal structure of the MID domain from a Piwi Argonaute and use docking experiments to show its ability to specify recognition of 5′ uridine (1U-bias) of piRNAs. Mutational analyses reveal the importance of 5′ end-recognition within the MID domain for piRNA biogenesis in vivo. Finally, domain-swapping experiments uncover an unexpected role for the MID-PIWI module of a Piwi protein in dictating the transposon strand-orientation of its bound piRNAs. Our work identifies structural features that allow distinguishing individual Piwi members during piRNA biogenesis.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
21.3
作者：
Kirino, Yohei;Kim, Namwoo;de Planell-Saguer, Mariangels;Khandros, Eugene;Chiorean, Stephanie;Klein, Peter S.;Rigoutsos, Isidore;Jongens, Thomas A.;Mourelatos, Zissimos
通讯作者：
Mourelatos, Zissimos
影响因子：
14.9
作者：
Davis IW;Leaver-Fay A;Chen VB;Block JN;Kapral GJ;Wang X;Murray LW;Arendall WB 3rd;Snoeyink J;Richardson JS;Richardson DC
通讯作者：
Richardson DC
影响因子：
3
作者：
Morris, Garrett M.;Huey, Ruth;Lindstrom, William;Sanner, Michel F.;Belew, Richard K.;Goodsell, David S.;Olson, Arthur J.
通讯作者：
Olson, Arthur J.
影响因子：
64.5
作者：
Mi, Shijun;Cai, Tao;Qi, Yijun
通讯作者：
Qi, Yijun