Natural variation in herbivore-induced volatiles in Arabidopsis thaliana.
Natural variation in herbivore-induced volatiles in Arabidopsis thaliana.
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DOI：
10.1093/jxb/erq127
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发表时间：
2010-06
影响因子：
6.9
通讯作者：
Bouwmeester HJ
中科院分区：
文献类型：
--
作者：
Snoeren TA;Kappers IF;Broekgaarden C;Mumm R;Dicke M;Bouwmeester HJ
为了研究拟南芥的自然变异是否可以从诱导挥发性物质排放的角度来剖析其对草食反应的遗传基础，本研究对9份材料进行了啃咬rapae毛虫和植物激素茉莉酸（jasmonic acid， JA）处理后的草食特征分析。对顶空73个化合物的分析表明，不同种质间个别化合物的排放速率存在定量差异。此外，在双选择实验中，当向寄生蜂提供几种组合的顶空挥发物时，JA处理后挥发性化合物的释放变化反映在它们的行为上。与植物挥发物排放相关的基因转录水平也不同。基因BSMT1和Cyp72A13分别与水杨酸甲酯和（E，E）-4,8,12-三甲基三萜-1,3,7,11-四烯（TMTT）的释放有关。总的来说，拟南芥在对草食的响应中释放的挥发性混合物方面表现出有趣的表型变化，可以利用这些变化来鉴定这一重要植物性状的基因和等位基因。
To study whether natural variation in Arabidopsis thaliana could be used to dissect the genetic basis of responses to herbivory in terms of induced volatile emissions, nine accessions were characterized upon herbivory by biting-chewing Pieris rapae caterpillars or after treatment with the phytohormone jasmonic acid (JA). Analysis of 73 compounds in the headspace showed quantitative differences in the emission rates of several individual compounds among the accessions. Moreover, variation in the emission of volatile compounds after JA treatment was reflected in the behaviour of the parasitoid Diadegma semiclausum when they were offered the headspace volatiles of several combinations of accessions in two-choice experiments. Accessions also differ in transcript levels of genes that are associated with the emission of plant volatiles. The genes BSMT1 and Cyp72A13 could be connected to the emission of methyl salicylate and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT), respectively. Overall, Arabidopsis showed interesting phenotypic variations with respect to the volatile blend emitted in response to herbivory that can be exploited to identify genes and alleles that underlie this important plant trait.
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影响因子：
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通讯作者：
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