Increased levels of multiresistant bacteria and resistance genes after wastewater treatment and their dissemination into lake geneva, Switzerland.
Increased levels of multiresistant bacteria and resistance genes after wastewater treatment and their dissemination into lake geneva, Switzerland.
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DOI：
10.3389/fmicb.2012.00106
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发表时间：
2012
影响因子：
5.2
通讯作者：
Bürgmann H
中科院分区：
文献类型：
--
作者：
Czekalski N;Berthold T;Caucci S;Egli A;Bürgmann H
目前，关于多重耐药细菌(MRB)及其耐药基因在自然水环境中的去向和持久性还知之甚少。瑞士洛桑市经过处理但部分未经处理的污水被排放到Vidy Bay(日内瓦湖)，导致该湖这部分地区污染严重。在目前的工作中，我们研究了洛桑污水中MRB和抗性基因的流行情况。对来自医院和城市未经处理的污水、洛桑污水处理厂(WTP)的经处理污水以及在WTP出水管道附近和饮用水水泵附近的偏远地点采集的湖水和沉积物样本进行了MRB流行情况的评估。对选定的菌株进行了鉴定(16S rRNA基因片段测序)，并对进一步的抗性、抗性基因和质粒进行了鉴定。对这一问题的研究大多依赖于以栽培为基础的方法。然而，这些工具的局限性是众所周知的，特别是对于环境微生物群落，与培养无关的分子工具应该并行应用，以便考虑到不可培养的生物体。在这里，我们使用TaqMan实时定量聚合酶链式反应直接对环境DNA提取物中的磺胺抗性基因sul1和sul2进行了定量。医院污水中MRB和抗生素耐药基因(ARGs)含量最高。废水处理使细菌总数减少了78%，但观察到了筛选极端多重耐药菌株和积累耐药基因的证据。我们的数据清楚地表明，污水处理厂排水口附近的沉积物受到了ARGs的污染。讨论了湖泊作为MRB水库的潜力和潜在的风险。
At present, very little is known about the fate and persistence of multiresistant bacteria (MRB) and their resistance genes in natural aquatic environments. Treated, but partly also untreated sewage of the city of Lausanne, Switzerland is discharged into Vidy Bay (Lake Geneva) resulting in high levels of contamination in this part of the lake. In the present work we have studied the prevalence of MRB and resistance genes in the wastewater stream of Lausanne. Samples from hospital and municipal raw sewage, treated effluent from Lausanne’s wastewater treatment plant (WTP) as well as lake water and sediment samples obtained close to the WTP outlet pipe and a remote site close to a drinking water pump were evaluated for the prevalence of MRB. Selected isolates were identified (16S rRNA gene fragment sequencing) and characterized with regards to further resistances, resistance genes, and plasmids. Mostly, studies investigating this issue have relied on cultivation-based approaches. However, the limitations of these tools are well known, in particular for environmental microbial communities, and cultivation-independent molecular tools should be applied in parallel in order to take non-culturable organisms into account. Here we directly quantified the sulfonamide resistance genes sul1 and sul2 from environmental DNA extracts using TaqMan real-time quantitative PCR. Hospital sewage contained the highest load of MRB and antibiotic resistance genes (ARGs). Wastewater treatment reduced the total bacterial load up to 78% but evidence for selection of extremely multiresistant strains and accumulation of resistance genes was observed. Our data clearly indicated pollution of sediments with ARGs in the vicinity of the WTP outlet. The potential of lakes as reservoirs of MRB and potential risks are discussed.
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影响因子：
11.8
作者：
Cattoir, Vincent;Poirel, Laurent;Aubert, Camille;Soussy, Claude-James;Nordmann, Patrice
通讯作者：
Nordmann, Patrice
影响因子：
3.3
作者：
Filippini, Massimo;Masiero, Giuliano;Moschetti, Karine
通讯作者：
Moschetti, Karine
DOI：
10.1080/09603120220129292
发表时间：
2002-06-01
影响因子：
3.2
作者：
Esiobu, N;Armenta, L;Ike, J
通讯作者：
Ike, J
影响因子：
4.2
作者：
da Silva, MF;Tiago, I;Manaia, CM
通讯作者：
Manaia, CM
影响因子：
11.4
作者：
Cummings, David E.;Archer, Karisa F.;Zuill, Douglas E.
通讯作者：
Zuill, Douglas E.