Augmentation of the Photoreactivation Gene in Fremyella diplosiphon Confers UV-B Tolerance.
Augmentation of the Photoreactivation Gene in Fremyella diplosiphon Confers UV-B Tolerance.
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DOI：
10.1021/acsomega.2c03938
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发表时间：
2022-10-04
期刊：
影响因子：
4.1
通讯作者：
Sitther, Viji
中科院分区：
文献类型：
--
作者：
Gichuki, Samson M.;Arumanayagam, Anithachristy S.;Tabatabai, Behnam;Yalcin, Yavuz S.;Wyatt, LaDonna;Sitther, Viji
尽管蓝细菌作为可再生能源具有巨大的潜力，但紫外线暴露的增加是其商业可行性和生产力的主要障碍。 Fremyella dilosiphon 是一种广泛研究的蓝细菌，由于其高脂质含量而具有巨大的生物燃料能力。为了增强该物种的紫外线胁迫耐受性，我们通过遗传转化在野生型双倍虹吸虫 (B481-WT) 中过度表达了编码光裂合酶 DNA 修复酶的光再激活基因 (phr A)。我们的努力产生了一种转化体（B481-ViAnSa），其 phr A mRNA 转录水平增加了 3808 倍，并且在 UV-B 胁迫下生长增强。此外，在紫外线辐射 12 和 16 小时后，转化体中的 DNA 链断裂显着降低，在辐射后 48 小时，B481-ViAnSa 中的 dsDNA 回收率 (98.1%) 显着高于 B481-WT (81.5%)。与野生型（72 小时）相比，转化体中光系统 II 的恢复时间显着缩短（48 小时）。对高价值脂肪酸甲酯 (FAME) 的评估显示，棕榈酸甲酯（十六碳烯酸甲酯 (C16:0)）是最主要的成分，占转化体中已鉴定 FAME 的 53.43%。该研究结果提供了一种增强蓝藻紫外线耐受性的有前景的方法，从而为商业生物燃料生产的大规模开放或封闭池塘培养铺平了道路。
In spite of the enormous potential of cyanobacteria as a renewable energy source, elevated UV exposure is a major impediment to their commercial viability and productivity. Fremyella diplosiphon is a widely explored cyanobacterium with great biofuel capacity due to its high lipid content. To enhance UV stress tolerance in this species, we overexpressed the photoreactivation gene (phr A) that encodes for photolyase DNA repair enzyme in the wild type F. diplosiphon (B481-WT) by genetic transformation. Our efforts resulted in a transformant (B481-ViAnSa) with a 3808-fold increase in the phr A mRNA transcript level and enhanced growth under UV-B stress. Additionally, DNA strand breaks in the transformant were significantly lower after 12 and 16 h of UV radiation, with significantly higher dsDNA recovery in B481-ViAnSa (98.1%) compared to that in B481-WT (81.5%) at 48 h post irradiation. Photosystem II recovery time in the transformant was significantly reduced (48 h) compared to that in the wild type (72 h). Evaluation of high-value fatty acid methyl esters (FAMEs) revealed methyl palmitate, the methyl ester of hexadecenoic acid (C16:0), to be the most dominant component, accounting for 53.43% of the identified FAMEs in the transformant. Results of the study offer a promising approach to enhance UV tolerance in cyanobacteria, thus paving the way to large-scale open or closed pond cultivation for commercial biofuel production.
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DOI：
10.1080/15567036.2018.1463310
发表时间：
2018-01-01
影响因子：
2.9
作者：
Kumar, Vinod;Nanda, Manisha;Chauhan, Pankaj K.
通讯作者：
Chauhan, Pankaj K.
DOI：
10.1016/b978-0-12-814667-5.00007-6
发表时间：
2019-01-01
期刊：
CYANOBACTERIA: FROM BASIC SCIENCE TO APPLICATIONS
影响因子：
--
作者：
Pathak, Jainendra;Ahmed, Haseen;Sinha, Rajeshwar P.
通讯作者：
Sinha, Rajeshwar P.
影响因子：
5.7
作者：
Rastogi, Rajesh P.;Singh, Shailendra P.;Sinha, Rajeshwar P.
通讯作者：
Sinha, Rajeshwar P.
影响因子：
1.5
作者：
Kokabi, Maryam;Yousefzadi, Morteza;Arman, Mitra
通讯作者：
Arman, Mitra
DOI：
10.1007/s40502-014-0084-9
发表时间：
2014-06-01
期刊：
Indian Journal of Plant Physiology
影响因子：
--
作者：
Apostolova, Emilia L.;Pouneva, Irina;Misra, Amarendra N.
通讯作者：
Misra, Amarendra N.