Quantitative genomics of aggressive behavior in Drosophila melanogaster.
Quantitative genomics of aggressive behavior in Drosophila melanogaster.
复制标题
果蝇中侵略性行为的定量基因组学。
DOI：
10.1371/journal.pgen.0020154
复制
发表时间：
2006-09-15
期刊：
影响因子：
4.5
通讯作者：
Mackay, Trudy F. C.
中科院分区：
文献类型：
--
作者：
Edwards, Alexis C.;Rollmann, Stephanie M.;Morgan, Theodore J.;Mackay, Trudy F. C.
攻击行为对于动物的生存和繁殖很重要，过度的攻击行为给人类社会带来了巨大的社会和经济负担。尽管生物胺在调节攻击行为中的作用已得到很好的表征，但影响这种复杂行为的其他遗传机制仍然难以捉摸。在这里，我们开发了一种快速量化果蝇攻击行为的测定方法，并生成了具有不同攻击水平的重复选择系。攻击行为的实际遗传力约为 0.10，对选择的表型反应特别影响攻击行为。我们使用全基因组表达分析，以 0.001 的错误发现率为 0.001 的错误发现率，识别了 1,539 个探针组，这些探针组在不同的选择系之间具有不同的表达水平。我们量化了在共同同基因背景下产生的候选基因中的 19 个突变的攻击行为，并鉴定了 15 个影响攻击行为的新基因。遗传分化系的表达谱是识别影响复杂性状的基因的有效策略。攻击性行为是一种复杂的特征，受到众多相互作用基因的影响，这些基因的表达取决于环境。在追求配偶、领土或食物时，攻击性可能是有选择的优势；然而，过度的攻击性可能是有害的。人类病态的攻击性给社会造成了巨大的负担。尽管生物胺系统的功能障碍通常与攻击行为的改变有关，但这仅代表攻击行为复杂遗传结构的“冰山一角”。果蝇果蝇是探索攻击行为遗传基础的优秀模型遗传系统。作者开发了一种快速测定方法来量化果蝇的攻击性，并用它来选择遗传上不同的复制品系，以增加和减少遗传异质基群体的行为。他们使用全基因组表达谱来识别这些品系之间基因表达的变异，并鉴定了选择品系之间存在差异的 1,539 个转录本，说明了攻击行为的复杂基因组基础。作者评估了 19 个基因突变的果蝇的攻击行为，这些基因通过差异转录本丰度分析而涉及，并确定了 15 个影响这一复杂性状的新候选基因，其中 8 个具有人类直系同源基因。
Aggressive behavior is important for animal survival and reproduction, and excessive aggression is an enormous social and economic burden for human society. Although the role of biogenic amines in modulating aggressive behavior is well characterized, other genetic mechanisms affecting this complex behavior remain elusive. Here, we developed an assay to rapidly quantify aggressive behavior in Drosophila melanogaster, and generated replicate selection lines with divergent levels of aggression. The realized heritability of aggressive behavior was approximately 0.10, and the phenotypic response to selection specifically affected aggression. We used whole-genome expression analysis to identify 1,539 probe sets with different expression levels between the selection lines when pooled across replicates, at a false discovery rate of 0.001. We quantified the aggressive behavior of 19 mutations in candidate genes that were generated in a common co-isogenic background, and identified 15 novel genes affecting aggressive behavior. Expression profiling of genetically divergent lines is an effective strategy for identifying genes affecting complex traits. Aggressive behavior is a complex trait affected by numerous interacting genes whose expression depends on the environment. Aggression can be selectively advantageous in the pursuit of mates, territory, or food; however, excessive aggression may be deleterious. Pathological levels of aggression in humans create an enormous burden to society. Although dysfunction of the biogenic amine systems is often associated with alterations in aggressive behavior, this represents only the “tip of the iceberg” of the complex genetic architecture of aggressive behavior. The fruit fly Drosophila melanogaster is an excellent model genetic system for exploring the genetic basis of aggressive behavior. The authors have developed a rapid assay to quantify Drosophila aggression, and have used it to select genetically divergent replicate lines for increased and decreased behavior from a genetically heterogeneous base population. They used whole-genome expression profiling to identify variation in gene expression among these lines, and identified 1,539 transcripts that differed between the selection lines, illustrating the complex genomic basis of aggressive behavior. The authors evaluated aggressive behavior of flies with mutations in 19 genes that were implicated by the analysis of differential transcript abundance, and identified 15 novel candidate genes affecting this complex trait, eight of which have human orthologs.
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影响因子：
2.6
作者：
JACOBS, ME
通讯作者：
JACOBS, ME
影响因子：
30.8
作者：
Anholt, RRH;Dilda, CL;Mackay, TFC
通讯作者：
Mackay, TFC
影响因子：
3.8
作者：
HEMMAT, M;EGGLESTON, P
通讯作者：
EGGLESTON, P
影响因子：
2.5
作者：
HOFFMANN, AA
通讯作者：
HOFFMANN, AA
影响因子：
56.9
作者：
Caspi, A;McClay, J;Poulton, R
通讯作者：
Poulton, R