Classification and quantification of leaf curvature.

Classification and quantification of leaf curvature.
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叶曲率的分类和量化

DOI:
10.1093/jxb/erq111
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发表时间:
2010-06
影响因子:
6.9
通讯作者:
He Y
He Y
中科院分区:
生物学1区
文献类型:
--
作者:
Liu Z;Jia L;Mao Y;He Y

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拟南芥在极性、细胞分裂和生长素反应方面缺陷的各种突变体的特征在于某些类型的叶片弯曲。然而,如果没有曲率的定量测量,比较曲率以澄清基因功能可能是困难的。在这里,一种新的方法进行分类和量化的叶片曲率的报告。从拟南芥突变体和叶片扁平度缺陷的转基因株系中选择了22个突变等位基因。根据叶片弯曲的方向、轴向、位置和程度对突变体进行了分类。基于叶片整体的整体度量和叶片四个区域的局部度量,提出了叶片曲率指数(CI)。CI值占的方向,轴,位置和程度的叶片弯曲在生长室中生长的所有拟南芥突变体。通过比较突变体间的CI值,揭示了叶片曲率的时空变化,反映了突变体等位基因的强弱和相应基因的活性。使用弯曲指数,复杂遗传背景中的弯曲程度变得定量且可比较,从而为定义叶片发育的遗传成分和培育具有有效捕获阳光进行光合作用和高产所需的叶片弯曲度的新品种提供了有用的工具。
Various mutants of Arabidopsis thaliana deficient in polarity, cell division, and auxin response are characterized by certain types of leaf curvature. However, comparison of curvature for clarification of gene function can be difficult without a quantitative measurement of curvature. Here, a novel method for classification and quantification of leaf curvature is reported. Twenty-two mutant alleles from Arabidopsis mutants and transgenic lines deficient in leaf flatness were selected. The mutants were classified according to the direction, axis, position, and extent of leaf curvature. Based on a global measure of whole leaves and a local measure of four regions in the leaves, the curvature index (CI) was proposed to quantify the leaf curvature. The CI values accounted for the direction, axis, position, and extent of leaf curvature in all of the Arabidopsis mutants grown in growth chambers. Comparison of CI values between mutants reveals the spatial and temporal variations of leaf curvature, indicating the strength of the mutant alleles and the activities of the corresponding genes. Using the curvature indices, the extent of curvature in a complicated genetic background becomes quantitative and comparable, thus providing a useful tool for defining the genetic components of leaf development and to breed new varieties with leaf curvature desirable for the efficient capture of sunlight for photosynthesis and high yields.
DOI: 10.2307/2656848
发表时间: 2000-05-01
影响因子: 3
作者:
Hay, JO;Moulia, B;Silk, WK
通讯作者: Silk, WK
DOI: 10.1007/s003440000004
发表时间: 2000-03-01
影响因子: 4.8
作者:
Moulia, B
通讯作者: Moulia, B
DOI: 10.1007/s004380050016
发表时间: 1999-06-01
期刊: MOLECULAR AND GENERAL GENETICS
影响因子: --
作者:
Serrano-Cartagena, J;Robles, P;Micol, JL
通讯作者: Micol, JL
DOI: 10.1073/pnas.0604349103
发表时间: 2006-08-29
影响因子: 11.1
作者:
White, Derek W. R.
通讯作者: White, Derek W. R.
DOI: 10.1006/dbio.1999.9443
发表时间: 1999-11-15
影响因子: 2.7
作者:
Donnelly, PM;Bonetta, D;Dengler, NG
通讯作者: Dengler, NG