The Arabidopsis DREB2 genetic pathway is constitutively repressed by basal phosphoinositide-dependent phospholipase C coupled to diacylglycerol kinase.
The Arabidopsis DREB2 genetic pathway is constitutively repressed by basal phosphoinositide-dependent phospholipase C coupled to diacylglycerol kinase.
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DOI：
10.3389/fpls.2013.00307
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发表时间：
2013
影响因子：
5.6
通讯作者：
Ruelland E
中科院分区：
文献类型：
--
作者：
Djafi N;Vergnolle C;Cantrel C;Wietrzyñski W;Delage E;Cochet F;Puyaubert J;Soubigou-Taconnat L;Gey D;Collin S;Balzergue S;Zachowski A;Ruelland E
磷酸肌苷依赖性磷脂酶C （pi - plc）在各种刺激下被激活。它们利用iii型磷脂酰肌醇-4激酶（PI4KIII）提供的底物产生肌醇三磷酸和二酰基甘油（DAG）， DAG激酶（DGKs）将DAG磷酸化为磷脂酸（PA）。pi4kii、pi - plc和DGKs在基础信号传导中的作用尚不清楚。研究了基础PI-PLC通路对拟南芥悬浮细胞基因表达的调控作用。转录组范围的分析可以鉴定出分别被edelfosine、30 μM wortmannin或R59022 （pi - plc、pi4kii和DGKs抑制剂）改变表达的基因。我们发现，对其中一种分子有反应的基因更有可能受到其他两种抑制剂的类似调节。这些药物的共同作用是抑制PA的形成，这表明基础pi - plc通过与DGKs的偶联在一定程度上影响基因表达。在抑制剂存在的情况下，悬浮细胞和幼苗中的一些DREB2基因被上调。DREB2基因编码的转录因子在对环境胁迫（包括脱水）的反应中起主要作用。它们与C-repeat基序结合，被称为干旱响应元件，在PI-PLC途径抑制剂上调的基因启动子中确实富集。磷脂酶D （PLDs）也可以产生PA。我们发现，被PI-PLC抑制剂上调的DREB2基因对PLD基础活性有正向或负向调节，或不受影响。我们的数据表明，DREB2遗传途径在静止条件下被组成性抑制，而DGK偶联PI-PLC在悬浮细胞和幼苗的这一过程中是活跃的。我们讨论了DREB2基因的这种基础负调控是如何与其应激触发的正调控兼容的。
Phosphoinositide-dependent phospholipases C (PI-PLCs) are activated in response to various stimuli. They utilize substrates provided by type III-Phosphatidylinositol-4 kinases (PI4KIII) to produce inositol triphosphate and diacylglycerol (DAG) that is phosphorylated into phosphatidic acid (PA) by DAG-kinases (DGKs). The roles of PI4KIIIs, PI-PLCs, and DGKs in basal signaling are poorly understood. We investigated the control of gene expression by basal PI-PLC pathway in Arabidopsis thaliana suspension cells. A transcriptome-wide analysis allowed the identification of genes whose expression was altered by edelfosine, 30 μM wortmannin, or R59022, inhibitors of PI-PLCs, PI4KIIIs, and DGKs, respectively. We found that a gene responsive to one of these molecules is more likely to be similarly regulated by the other two inhibitors. The common action of these agents is to inhibit PA formation, showing that basal PI-PLCs act, in part, on gene expression through their coupling to DGKs. Amongst the genes up-regulated in presence of the inhibitors, were some DREB2 genes, in suspension cells and in seedlings. The DREB2 genes encode transcription factors with major roles in responses to environmental stresses, including dehydration. They bind to C-repeat motifs, known as Drought-Responsive Elements that are indeed enriched in the promoters of genes up-regulated by PI-PLC pathway inhibitors. PA can also be produced by phospholipases D (PLDs). We show that the DREB2 genes that are up-regulated by PI-PLC inhibitors are positively or negatively regulated, or indifferent, to PLD basal activity. Our data show that the DREB2 genetic pathway is constitutively repressed in resting conditions and that DGK coupled to PI-PLC is active in this process, in suspension cells and seedlings. We discuss how this basal negative regulation of DREB2 genes is compatible with their stress-triggered positive regulation.
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影响因子：
7.4
作者：
Boss, Wendy F.;Sederoff, Heike Winter;Perera, Imara Y.
通讯作者：
Perera, Imara Y.
影响因子：
4.8
作者：
Gómez-Merino, FC;Arana-Ceballos, FA;Mueller-Roeber, B
通讯作者：
Mueller-Roeber, B
影响因子：
6.9
作者：
Garwe, D;Thomson, JA;Mundree, SG
通讯作者：
Mundree, SG
水稻二酰基甘油激酶家族：使用瞬态RNA干扰的功能分析。
影响因子：
5.6
作者：
Ge H;Chen C;Jing W;Zhang Q;Wang H;Wang R;Zhang W
通讯作者：
Zhang W
影响因子：
4.4
作者：
Gómez-Porras JL;Riaño-Pachón DM;Dreyer I;Mayer JE;Mueller-Roeber B
通讯作者：
Mueller-Roeber B