Arsenic sulfide induces miR-4665-3p to inhibit gastric cancer cell invasion and migration
Arsenic sulfide induces miR-4665-3p to inhibit gastric cancer cell invasion and migration
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硫化砷诱导miR-4665-3p抑制胃癌细胞侵袭和迁移
DOI：
10.2147/dddt.s209219
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
Chen Siyu
中科院分区：
文献类型：
--
作者：
Zhang Xiuli;Tan Zhen;Kang Ting;Zhu Chuanying;Chen Siyu
目的胃癌的发生是一个多步骤的过程，是世界上第二大癌症死亡原因，具有很高的侵袭和转移率。MicroRNAs （miRNAs）与控制转录组的机制进行复杂的相互作用，并同时靶向多个mrna。最近的证据表明，mirna参与癌症的进展，包括促进细胞周期，赋予细胞凋亡抗性，增强侵袭性和转移。在这里，我们旨在阐明mirna，特别是microRNA-4665-3p （miR-4665-3p）在胃癌（GC）中硫化砷抑制作用中的作用。方法采用miRNA芯片检测砷硫化物诱导AGS细胞miRNA表达的变化。RT-PCR进一步验证了GC组织中硫化砷调控的mirna。通过伤口愈合实验和transwell实验检测miR-4665-3p对胃癌细胞迁移和侵袭的抑制作用。Western blot检测EMT相关蛋白的表达以及miR-4665-3p的推测靶点。结果硫化砷上调miR-4665-3p，抑制胃癌细胞的迁移和侵袭。MiRBase和Western blotting结果显示，miR-4665-3p直接下调癌蛋白GSE1。形态学观察也表明，上调miR-4665-3p可抑制GC细胞的EMT。结论砷诱导的miR-4665-3p表达升高可抑制胃癌细胞的侵袭、转移和EMT，有可能成为胃癌治疗的新靶点。
Purpose Gastric carcinogenesis is a multistep process and is the second-highest cause of cancer death worldwide with a high incidence of invasion and metastasis. MicroRNAs (miRNAs) engage in complex interactions with the machinery that controls the transcriptome and concurrently target multiple mRNAs. Recent evidence has shown that miRNAs are involved in the cancer progression, including promoting cell-cycle, conferring resistance to apoptosis, and enhancing invasiveness and metastasis. Here, we aim to elucidate the roles of miRNAs, especially microRNA-4665-3p (miR-4665-3p), in the inhibitory effect of arsenic sulfide in gastric cancer (GC). Methods The arsenic sulfide-induced miRNA expression alterations in AGS cells was determined by miRNA microarray. RT-PCR was used to further verify the arsenic sulfide-regulated miRNAs in GC tissues. The inhibition of miR-4665-3p on the migration and invasion of GC cells were determined by wound healing assay and transwell assay. Western blot analysis was used to detect the expression of EMT related proteins and the putative target of miR-4665-3p. Results The miR-4665-3p was up-regulated by arsenic sulfide and showed inhibition upon the migration and invasion of GC cells. MiRBase and Western blotting indicated that miR-4665-3p directly down-regulated the oncoprotein GSE1. Morphological observation also indicated that the up-regulation of miR-4665-3p inhibits the EMT in GC cells. Conclusion Our data demonstrates that the increased expression of miR-4665-3p induced by arsenic sulfide suppresses the cell invasion, metastasis and EMT of GC cells, and has the potential to be a novel therapeutic target in GC.
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DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
3
作者：
Lee SH;Lee SJ;Jin SM;Lee NH;Kim DH;Chae SW;Sohn JH;Kim WS
通讯作者：
Kim WS
影响因子：
11.5
作者：
Yu, XM;Lo, CY;Luk, JM
通讯作者：
Luk, JM
DOI：
10.1016/j.bbrc.2016.01.168
发表时间：
2016-02-26
影响因子：
3.1
作者：
Chai, Peng;Tian, Jingzhong;Liu, Bin
通讯作者：
Liu, Bin
中药雄黄的主要成分硫化砷在体外和体内诱导胃癌细胞凋亡
DOI：
10.2147/dddt.s74379
发表时间：
2015
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Zhang L;Tian W;Kim S;Ding W;Tong Y;Chen S
通讯作者：
Chen S